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Thylakoid polypeptides of light and dark aged chloroplasts.

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Spinach (Spinacia oleracea) chloroplasts were aged at 4 degrees C under red light and in the dark. The electron transport activity was monitored together with the thylakoid polypeptide patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The light-induced decay of photosystem II

Renal albumin handling: a look at the dark side of the filter.

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Renal albumin handling is of major interest because albuminuria is an important risk factor for reno-cardiovascular diseases. In this issue a challenging study attempts to shift the paradigm of very low fractional albumin filtration and degradation in proximal tubule cells. The conclusions are of

Primary biliary cirrhosis, dark adaptometry, electro-oculography, and vitamin A state.

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Twenty five patients with primary biliary cirrhosis were studied for vitamin A state. In nine patients found to have low circulating vitamin A concentrations no abnormality was found on electro-oculography or in dark adaptation. A positive correlation was found between retinol binding protein and

Bilirubin-induced cell death during continuous and intermittent phototherapy and in the dark.

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OBJECTIVE To compare continuous and intermittent light exposure in the presence of bilirubin with respect to cellular damage. Furthermore, it was of interest to characterize the nature of cellular toxicity of bilirubin in the dark. METHODS A murine lymphoma cell line, L5178Y-R (LY-R), was exposed to

Children's consumption of dark green, leafy vegetables with added fat enhances serum retinol.

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A randomized, double-blind, controlled study was conducted to determine whether the consumption of leafy vegetables by preschool children would enhance their serum vitamin A concentration to acceptable levels. Preschool children (n = 519; 2.5-6 y) in Saboba, northern Ghana, were randomly assigned to

Isolation and characterization of an acyl-CoA thioesterase from dark-grown Euglena gracilis.

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An acyl-CoA hydrolase from dark-grown Euglena gracilis Z was purified 700-fold by subjecting the 105,000g supernatant of the cell-free extract to (NH4)2SO4 precipitation, acid precipitation, calcium phosphate gel treatment, gel filtration on Sephadex G-100, and chromatography on QAE-Sephadex,

The density of apical cells of dark-grown protonemata of the moss Ceratodon purpureus.

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Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First,

Dark Field Microscopy-Based Biosensors for the Detection of E. coli in Environmental Water Samples.

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Development of sensitive methods for the determination of E. coli bacteria contamination in water distribution systems is of paramount importance to ensure the microbial safety of drinking water. This work presents a new sensing platform enabling the fast detection of bacteria in field

All Cats are Gray in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma.

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In veterinary medicine, assay performance is often affected by the lack of species-specific diagnostic tools. Reliable biomarkers might be identified by investigating biological fluids of the species of interest, but protein sequence databases are often incomplete and human-specific devices for

Disrupted daily light-dark cycle induces the expression of hepatic gluconeogenic regulatory genes and hyperglycemia with glucose intolerance in mice.

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We elucidated associations between metabolic disorders and the environmental light-dark (LD) cycle that entrains the circadian clock located in the suprachiasmatic nucleus of mammals. Mice were fed with a high-fat/high-sucrose diet for eight weeks under a normal 12h light-12h dark cycle (LD 12:12)

Rainbow trout eyed-stage embryos demonstrate melatonin rhythms under light-dark conditions as measured by a newly developed time-resolved fluoroimmunoassay.

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Melatonin contents in eyed-stage embryos of rainbow trout (Oncorhynchus mykiss) were measured by a newly developed, highly sensitive, and nonisotopic immunoassay method: time-resolved fluoroimmunoassay (TR-FIA). The melatonin-bovine serum albumin (BSA) conjugate was immobilized by physical

Alpha-1-acid glycoprotein concentrations and protein binding of propranolol in Sprague-Dawley and Dark Agouti rat strains treated by phenobarbital.

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Propranolol binding to rat serum samples was studied after 7 days of phenobarbital (PB) administration to induce alpha-1-acid glycoprotein (AAG). In male Sprague-Dawley rats, serum AAG concentrations and the bound/free propranolol ratio (B/F) were increased slightly after enzyme-inducing drug

Photosynthetic apparatus in chilling-sensitive plants. VII. Comparison of the effect of galactolipase treatment of chloroplasts and cold-dark storage of leaves on photosynthetic electron flow.

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1. Both galactolipase treatment of tomato chloroplasts and the cold and dark storage of leaves induce a large degradation of chloroplast monogalactosyl diacylglycerol and digalactosyl diacylglycerol as well as an accumulatwon of free fatty acids accompanied by the inhibition of Hill reaction

Postmortal diffusion of plasma albumin in rat brain.

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After i.v. injection of Evans blue solution (EB), the postmortal diffusion of plasma albumin and its relationship to the postmortal interval (0 to 48 h) were studied in rat brain. A decrease in intravascular plasma albumin with increasing evidence of the same fraction in relation to the length of

Transformation of albumin into melanin by hydroxyl radicals.

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Bovine serum albumin (BSA) was exposed to hydroxyl radicals generated by the Fenton reaction. The reaction of the BSA solution with hydroxyl radicals resulted in a colour change from clear transparent to dark brown. The reaction was followed spectrophometrically. It was observed that during the

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