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Premature dissolution of the microsporocyte callose wall causes male sterility in transgenic tobacco.

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Male sterility in a petunia cytoplasmic male sterile line has been attributed to the early appearance of active callase, a beta-1,3-glucanase, in the anther locule. This leads to premature dissolution of the callose walls surrounding the microsporogenous cells. We have mimicked this aspect of the

Tapetum-specific expression of the gene for an endo-beta-1,3-glucanase causes male sterility in transgenic tobacco.

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A cDNA for a pathogenesis-related endo-beta-1,3-glucanase isolated from soybean, was fused to an anther tapetum-specific promoter (Osg6B promoter) isolated from rice and the resulting chimeric gene was introduced into tobacco. The Osg6B promoter became active in the anther tapetum during formation

Two similar but distinct second intron fragments from tobacco AGAMOUS homologs confer identical floral organ-specific expression sufficient for generating complete sterility in plants.

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The carpel- and stamen-specific AtAGIP promoter derived from the Arabidopsis AGAMOUS (AG) second intron/enhancer is ideal for engineering complete sterility but it is highly host-specific. To ascertain whether a chimeric promoter with similar tissue specificity can be created for species other than

Autonomy of Cytoplasmic Male Sterility in Grafted Scions of Tobacco.

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Negative results were obtained in experiments designed to detect graft transmission to progeny of cytoplasmic male sterility in tobacco, contrary to positive results reported by Frankel in petunia. Attempts to show graft transmission of male fertility also failed. Evidently transmission of

Induced abortions, miscarriages, and tobacco smoking as risk factors for secondary infertility.

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OBJECTIVE The aim was to determine whether induced abortions could increase the risk of secondary infertility. METHODS This was a case-control study; cases were women with secondary infertility, individually matched to two controls who were currently pregnant. Each participant was interviewed by one

The complete mitochondrial genome sequence of Sua-type cytoplasmic male sterility of tobacco (Nicotiana tabacum).

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To uncover the cytoplasmic male sterility (CMS)-associated mitochondrial genes of tobacco (Nicotiana tabacum), we determined the complete nucleotide sequence of Sua-CMS mitochondrial genome. The Sua-CMS mtDNA sequence is 522,731 bp in length and contains 34 protein-coding genes, 25 transfer RNA

Expression of kenaf mitochondrial chimeric genes HM184 causes male sterility in transgenic tobacco plants.

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Chimeric genes resulting from the rearrangement of a mitochondrial genome were generally thought to be a causal factor in the occurrence of cytoplasmic male sterility (CMS). In the study, earlier we reported that identifying a 47 bp deletion at 3'- flanking of atp9 that was linked to male sterile

[Effect of tobacco consumption on the spermatogenesis in males with idiopathic infertility].

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OBJECTIVE Our objective was to relate the tobacco with seminal spermatogenic parameters such as sperm morphology, concentration of spermatozoids and germinal cells in samples of semen from men with idiopatic infertility. METHODS A prospective study was carried out on a population of 131 men with

[Tobacco and male infertility: a retrospective study in infertile couples].

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BACKGROUND Different studies have shown an association between smoking habits and the presence of abnormal spermograms. However these reports have not been consistent throw out the literature. OBJECTIVE To analyze the effects of cigarette consumption in semen parameters from men attending our

Infertility, Pregnancy Loss and Adverse Birth Outcomes in Relation to Maternal Secondhand Tobacco Smoke Exposure.

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A substantial proportion of the etiology involved in female infertility and adverse pregnancy outcomes remains idiopathic. Recent scientific research has suggested a role for environmental factors in these conditions. Secondhand tobacco smoke (STS) contains a number of known or suspected

Relationship between semen quality and tobacco chewing in men undergoing infertility evaluation.

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OBJECTIVE Male fertility is affected by a variety of lifestyle habits that include tobacco use. A large population of Indian men is addicted to tobacco chewing. The objective of our study was to assess the relationship between tobacco chewing in these Indian men--who were part of an infertile

Tobacco use, alcohol consumption and infertility.

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An epidemiological study of the association between alcohol consumption, tobacco use and subfecundity is presented. Study subjects were recruited for a case-control study whose primary objective was to examine the association between occupational exposures and subfecundity. All 1069 women treated

A study of morphology, cytology and sterility in interspecific hybrids and amphidiploids of Nicotiana knightiana X N. umbratica.

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Interspecific hybrids and amphidiploids of Nicotiana knightiana Goodspeed (n= 12)x N. umbratica Burbidge (n = 23) resembled either parent in some characters and were intermediate in other characters. The F1 hybrids (2n = 35) showed mostly univalents during meiosis, while the amphidiploids (2n = 70)

A cytoplasmic male sterility-associated mitochondrial protein causes pollen disruption in transgenic tobacco.

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In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen development is a consequence of mitochondrial dysfunction. To provide definitive evidence that expression

Cytoplasmic male sterility is associated with large deletions in the mitochondrial DNA of two Nicotiana sylvestris protoclones.

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Two cytoplasmic male-sterile plants (CMSI and CMSII) were obtained by protoplast culture in Nicotiana sylvestris. Both plants showed large deletions (up to 50 kb) in their mitochondrial DNA. Restriction maps of the reorganized regions suggested that the deletions occurred via two homologous

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