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Bladzijde 1 van 17 resultaten
Role of invertase activity in processing quality of potatoes: Effect of storage temperature and duration.
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Invertase activity and processing attributes of three potato cultivars were studied to find the reason for deterioration of processing quality during their prolonged storage in commercial cold stores (4°C) as compared to elevated temperature storage (12 ± 0.5°C), with CIPC
Cloning and sequence analysis of the gene encoding invertase (INV1) from the yeast Candida utilis.
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The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence comparisons between yeast invertases. The cloned gene was sequenced and found to encode a polypeptide
THE PEPTASE, LIPASE, AND INVERTASE OF HEMOLYTIC STREPTOCOCCUS.
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1. A method has been outlined by which the enzymes of hemolytic streptococcus may be extracted with comparative ease. 2. The peptolytic enzyme is active between pH 4.4 and 8.7 with an optimum action at pH 7.2. It is destroyed in neutral phosphate solution at a temperature of 57 degrees C. continued
Differential expression of acid invertase genes during seed germination in Arabidopsis thaliana.
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In Arabidopsis thaliana (L.) Heynh ecotype Landsberg, levels of soluble acid invertase activity are closely related to the progress of seed germination. To study the mechanism(s) of the development of these enzymes, two cDNA clones that encode putative vacuolar acid invertases were isolated from
Invertases involved in the development of the parasitic plant Phelipanche ramosa: characterization of the dominant soluble acid isoform, PrSAI1.
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Phelipanche ramosa L. parasitizes major crops, acting as a competitive sink for host photoassimilates, especially sucrose. An understanding of the mechanisms of sucrose utilization in parasites is an important step in the development of new control methods. Therefore, in this study, we characterized
Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1): A Potential Candidate for Reducing Pre- and Post-harvest Loss of Sucrose in Sugarcane.
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In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play a key role in
The Maize Invertase-Deficient miniature-1 Seed Mutation Is Associated with Aberrant Pedicel and Endosperm Development.
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Genetic evidence is presented to show that the developmental stability of maternal cells in the pedicel at the base of maize seeds is determined by the genotype of the developing endosperm. An early degeneration and withdrawal of maternal cells from the endosperm of homozygous miniature (mn mn) seed
The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source.
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A 52-kDa protein, CEL3, has been separated from the culture filtrate of Agaricus bisporus during growth on cellulose. A PCR-derived probe was made, with a degenerate oligodeoxynucleotide derived from the amino acid sequence of a CEL3 CNBr cleavage product and was used to select cel3 cDNA clones from
Rs-198 Liquid Biofertilizers Affect Microbial Community Diversity and Enzyme Activities and Promote Vitis vinifera L. Growth
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Chemical fertilizers were applied on perennial tree vines to obtain high yields, which have resulted in considerable deterioration of soil quality, and it is likely to have negative impacts on the development of the grape industry. In this study, P. putida Rs-198 liquid biofertilizer
Translucent Tissue Defects in Solanum tuberosum L: I. Alterations in Amyloplast Membrane Integrity, Enzyme Activities, Sugars, and Starch Content.
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Kennebec (cv) potatoes randomly developed translucent areas in their centrally located pith-parenchymal cells during storage. These defective areas were characterized as having reduced starch concentration and increased levels of free sugars (i. e. sucrose and glucose) and inorganic phosphate.
Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424.
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Cell wall inulinase (EC 3.2.1.7) was purified from Kluyveromyces marxianus var. marxianus (formerly K. fragilis) and its N-terminal 33-amino acid sequence was established. PCR amplification of cDNA with 2 sets of degenerate primers yielded a genomic probe which was then used to screen a genomic
[Alkaline phosphatase in the intestinal mucosa of children with the malabsorption syndrome].
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110 children suffering from malabsorption underwent several biopsies of the gut to confirm coeliac disease (CD) following the ESPGAN criteria. We studied the values for alkaline phosphatase (AP) in the intestinal mucosa after gluten challenge. In 42 patients the after challenge biopsy was normal,
Minimization of post-harvest sucrose losses in drought affected sugarcane using chemical formulation.
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Sugarcane is a significant crop for production of sugar and ethanol in the world. In present perspective, drought is one of the frequently occurring abiotic stresses hampering the productivity of sugarcane causing heavy losses in sugar recovery. Post-harvest sugarcane deterioration attains more
[Effect of fibrous root extract of Coptis chinensis on soil microbes and enzyme activities].
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Coptis chinensis is widely used as Chinese medicine herbs and serious soil problems occur after continual cultivation of this medicinal plant. In the preset experiment, fibrous root extract of C. chinensis (REC) was added into soil to study the effect of REC on microbes and enzyme activity in soil.
Identification of Plasmopara viticola genes potentially involved in pathogenesis on grapevine suggests new similarities between oomycetes and true fungi.
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Plant diseases caused by fungi and oomycetes result in significant economic losses every year. Although phylogenetically distant, these organisms share many common features during infection. We identified genes in the oomycete Plasmopara viticola that are potentially involved in pathogenesis in
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