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isocitrate lyase/nicotiana
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Regulation of expression of the cucumber isocitrate lyase gene in cotyledons upon seed germination and by sucrose.
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A 6.5 kb cucumber genomic DNA fragment containing the icl gene was introduced into Nicotiana plumbaginifolia and shown to direct isocitrate lyase (ICL) mRNA synthesis in transgenic seedlings upon germination, in a temporally regulated manner. Two putative icl promoter fragments, of 2900 and 572 bp,
Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene.
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Recent data have shown that distinct DNA sequence elements direct the germination and sugar responses of the cucumber (Cucumis sativus, L.) malate synthase (Ms) gene (Sarah et al. (1996) Mol. Gen. Genet., 250, 153-161). Such information is, however, lacking for the isocitrate lyase (Icl) gene which
Knock-down of heat-shock protein 90 and isocitrate lyase gene expression reduced root-knot nematode reproduction.
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Crop losses caused by nematode infections are estimated to be valued at USD 157 billion per year. Meloidogyne incognita, a root-knot nematode (RKN), is considered to be one of the most important plant pathogens due to its worldwide distribution and the austere damage it can cause to a large variety
Synthesis of Glycolate from Pyruvate via Isocitrate Lyase by Tobacco Leaves in Light.
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Tobacco (Nicotiana tabacum var Havana Seed) leaf discs were supplied tracer quantities of [2-(14)C]- and [3-(14)C]pyruvate for 60 minutes in steady state photosynthesis with 21% or 1% O(2), and the glycolate oxidase inhibitor alpha-hydroxy-2-pyridinemethanesulfonic acid was then added for 5 or 10
Distinct cis-acting elements direct the germination and sugar responses of the cucumber malate synthase gene.
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The malate synthase gene (ms) promoter in cucumber (Cucumis sativus L.) was investigated with the aim of distinguishing DNA sequences mediating regulation of gene expression by sugar, and expression following seed germination. Promoter deletions were constructed and their ability to direct
Recognition of Peroxisomes by Immunofluorescence in Transformed and Untransformed Tobacco Cells.
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We report the visualization of peroxisomes in tobacco (Nicotiana tabacum) leaves using fluorescently labeled antibodies to glycolate oxidase. In transgenic tobacco leaves the expression of isocitrate lyase was also visualized. In dual probing experiments both enzymes were shown to be present
Ectopic expression of CaRLK1 enhances hypoxia tolerance with increasing alanine production in Nicotiana spp.
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In a previous report, the pepper receptor-like kinase 1 (CaRLK1) gene was shown to be responsible for negatively regulating plant cell death caused by pathogens via accumulation of superoxide anions. Here, we examined whether this gene also plays a role in regulating cell death under abiotic stress.
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