phytohaemagglutinin/borstkanker

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Phytohaemagglutinin responsiveness of peripheral lymphocytes and survival in patients with primary breast cancer.

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The response of peripheral blood lymphocytes from 153 breast cancer patients to the T-cell mitogen phytohaemagglutinin (PHA) was measured prior to surgery and patients assigned to either high (Hi/PHA) or low (Lo/PHA) PHA response groups. Patients were followed up for between 8 and 12 years, or until

[Reactivity of lymphocytes from patients with breast cancer to phytohaemagglutinin (author's transl)].

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Precipitation of serum and tissue proteins by phytohaemagglutinin (PHA) in breast cancer patients.

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Decreased production of soluble interleukin 2 receptor by phytohaemagglutinin-stimulated peripheral blood mononuclear cells in patients with breast cancer after adjuvant therapy.

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Suppression of in vitro peripheral blood lymphocyte mitogenesis by cytotoxic drugs commonly used in the treatment of breast cancer: a comparative study.

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Cultures of normal donor peripheral blood mononuclear cells were tested in vitro for suppression by chemotherapeutic agents or their metabolites. The drugs tested were those commonly used in the treatment of breast cancer, namely, 5-fluorouracil, doxorubicin, vincristine, methotrexate and

Effect of treatment on the immunological status of women with advanced breast cancer.

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An immunological profile has been serially studied in 72 patients with advanced breast cancer during the course of a randomized trial of chemotherapy and hormonal manipulation. DNCB+ patients were more likely to respond to either therapy, but no other test was predictive of response. In the

Deficient DNA repair capacity, a predisposing factor in breast cancer.

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Women with breast cancer and a family history of breast cancer and some with sporadic breast cancer are deficient in the repair of radiation-induced DNA damage compared with normal donors with no family history of breast cancer. DNA repair was measured indirectly by quantifying chromatid breaks in

Long-term effects on the immune system following local radiation therapy for breast cancer. 4. Proliferative responses and induction of suppressor activity of the blood lymphocyte population.

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The long-term effect of local irradiation for breast cancer on the blood lymphocyte population was examined in 149 women who had been disease-free for 5-6 and 10-11 years. The patients were included in a clinical trial aiming at determining the value of pre- and post-operative irradiation (45 Gy)

Enhanced viral inhibition of lymphocyte mitogenesis in patients with advanced breast cancer.

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Virus particles are frequently able to non-specifically inhibit the capacity of human peripheral blood lymphocytes to respond to mitogenic or antigenic stimuli. In the case of breast cancer patients with advanced disease, the quantity of virus required to abrogate responsiveness to

New SCM (structuredness of the cytoplasmatic matrix)-based approach in breast cancer detection.

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OBJECTIVE We evaluate the possibility to use a combination of techniques such as lymphocyte stimulation and the Cell Scan Instrument for early detection of breast cancer. This method can detect differences in lymphocytes activation in the presence of absence of cancer. METHODS The Cell Scan is a

Reduced mitogenic stimulation of peripheral blood mononuclear cells as a prognostic parameter for the course of breast cancer: a prospective longitudinal study.

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Immunosuppression has been often associated with the course of malignant diseases. In the present study, the proliferation of peripheral blood mononuclear cells (PBMCs) in response to mitogenic stimulation with phytohaemagglutinin (PHA) was assessed prospectively in 90 patients with stage I-III

Topoisomerase IIα levels and G2 radiosensitivity in T-lymphocytes of women presenting with breast cancer.

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Previous studies from our laboratory have identified a link between intracellular topoisomerase IIα (topo IIα) levels and chromosomal radiosensitivity, as measured by the frequencies of chromatid breaks in the so-called G2-assay. Lower topo IIα levels were associated with reduced chromosomal

Lymphocyte reactivity to mitogens and tumor sex steroid receptors in breast cancer patients.

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In 60 breast cancer patients in stages I and II the blastogenic transformation of peripheral blood lymphocytes after phytohaemagglutinin (PHA) pokeweed mitogen (PWM) and concanavalin A (Con A) stimulation were assayed and estrogen (ER) and progesterone (PgR) receptor concentrations in tumor cytosol

Establishment of a heterotypic 3D culture system to evaluate the interaction of TREG lymphocytes and NK cells with breast cancer.

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Three-dimensional (3D) culture approaches to investigate breast tumour progression are yielding information more reminiscent of the in vivo microenvironment. We have established a 3D Matrigel system to determine the interactions of luminal phenotype MCF-7 cells and basal phenotype MDA-MB-231 cells

New insights of CTLA-4 into its biological function in breast cancer.

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CTLA-4 is a negative regulator of the proliferation and the effector function of T-cells. Therefore, it might be important to determine its expression on tumor cells and T-lymphocytes from cancer patients, to investigate its role in initiating and maintaining the neoplastic pathogenesis. CTLA-4

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