The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla atrosanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O.sub.2.sup.- scavenging activity remains same before and after autoclaving; scavenges O.sub.2.sup.- from sub-zero temperature of -20.degree. C. to high temperature of +80.degree. C.; O.sub.2.sup.- scavenging activity at 25.degree. C. for 30 days without adding any stabilizing agent such as polyols or sugars; O.sub.2.sup.- scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4.degree. C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0.degree. C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53.times.10.sup.4 % per nmol per min at 0.degree. C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

Council of Scientific and Industrial Research

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD in cosmetic, food and pharmaceutical compositions

The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla atrosanguinea Lodd. Var. areyrophylla, said isozyme having the following characteristics, O.sub.2.sup.- scavenging activity remains same before and after autoclaving; scavenges O.sub.2.sup.- from sub-zero temperature of -20.degree. C. to high temperature of +80.degree. C.; O.sub.2.sup.- scavenging activity at 25.degree. C. for 30 days without adding any stabilizing agent such as polyols or sugars; O.sub.2.sup.- scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4.degree. C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0.degree. C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53.times.10.sup.4% per nmol per min at 0.degree. C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

Council of Scientific &amp; Industrial Research

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD and use of the said SOD in cosmetic, food, and pharmaceutical compositions

The invention relates to the use of an extract of the plant Potentilla erecta in the field of cosmetics and pharmacy, especially in dermatology. The invention more particularly relates to any application in which it is sought to reinforce the dermo-epidermal junction or to improve hair condition, by the improvement of the synthesis of collagen VII by keratinocytes and/or fibroblasts. In particular, these applications concern toning of the skin, diminishing of wrinkles or hair care. The invention also relates to a novel method of culture of cells, in particular of human fibroblasts or keratinocytes, for promoting the formation of collagen VII.

potentilla acaulis/cosmetica

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD in cosmetic, food and pharmaceutical compositions

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD and use of the said SOD in cosmetic, food, and pharmaceutical compositions

Use of potentilla erecta extract in the cosmetic and pharmaceutical field

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