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Bladzijde 1 van 41 resultaten
Structure of grafted crown-gall teratoma shoots of tobacco: Regulation of transformed cells.
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Crown-gall teratomas are tumors of higher plants with an intrinsic capacity for organogenesis. The growth pattern of tobacco (Nicotiana tabacum L.) teratoma shoots, which is highly aberrant in primary tumors, becomes normal when the shoots are grafted to healthy stock plants. However, certain
Suppression of the neoplastic state with the acquisition of specialized functions in cells, tissues, and organs of crown gall teratomas of tobacco.
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The neoplastic state in cells of tissues and organs that develop from cloned lines of crown gall teratomas of tobacco may be completely but reversibly suppressed. Stems and leaves found on teratoma shoots may appear morphologically normal and such organs contain all of the specialized cell types and
Biotransformation of nicotine alkaloids by tobacco shooty teratomas induced by a Ti plasmid mutant.
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Tobacco shooty or rooty teratomas and hairy roots were induced by Agrobacterium tumefaciens (pGV 3845), A. tumefaciens (pGV 3304) and A. rhizogenes (pRi 8196), respectively. The tobacco alkaloids, nicotine, nornicotine and anatabine, were produced in hairy roots and in rooty teratomas but not in
Auxin-facilitated utilization of glutamic acid by tobacco crown-gall teratoma cells.
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Teratoma tissues obtained by inoculating Nicotiana tabacum cv. "Turkish" with a moderately virulent strain of the crown-gall bacterium require the synthetic auxin, α-naphthaleneacetic acid (NAA) when glutamic acid is used as a sole nitrogen source in the culture medium. In contrast, growth on
Separate auxin- and cation-dependent mechanisms for glutamate utilization by normal and crown-gall teratoma cells of tobacco in culture.
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Crown-gall teratoma tissues of tobacco, when grown in culture, require exogeneous auxin (α-naphthaleneacetic acid) or high concentrations of K(+) in the medium to utilize ammonium glutamate as a nitrogen source. These factors are not required to utilize NO 3 (-) or glutamine. The effects of K(+) and
Temperature-sensitive expression of auxin-autotrophy by crown-gall teratoma cells of tobacco.
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Crown-gall teratoma tissues of tobacco (Nicotiana tabacum L.) grow in culture at 25° in the absence of added auxin or cell-division factors. The capacity of these tissues to grow without added auxin is temperature sensitive. At 35° auxin provided as α-naphthalene acetic acid or
Tumor and teratoma induction in tobacco plants by debudding.
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Stem and root tumors and teratomata may be induced in tobacco plants (Nicotiana tabacum L., var. One Sucker) by total debudding. Intact plants or plants only decapitated produced no tumors or teratomata throughout their life time. These findings suggest a possibly important oncological relationship
Auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of Agrobacterium tumefaciens.
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We have studied the mechanism of auxin autonomy in tobacco (Nicotiana tabacum L.) crowngall tissues transformed by the auxin-mutant (tms (-)) A66 strain of Agrobacterium tumefaciens. Normally, tms (-) tobacco tumor tissues require the formation of shoots to exhibit auxin-independent growth in
Regulation of phenotypic expression in crown-gall teratoma tissues of tobacco.
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Total fatty acids of habituated and teratoma tissue cultures of tobacco.
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Isoenzymes of acid phosphatase and non-specific esterases in cultures of neoplastic and normal tobacco tissues.
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Axenic cultures of normal, habituated and crown gall teratoma were grown under varying conditions to examine the effects of environment on the expression of neoplastic character. Acid phosphatase patterns on polyacrylamide gels did not vary greatly among tissues although there were differences in
Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine.
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Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate
Ribosylzeatin and zeatin in tobacco crown gall tissue.
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Cytokinins were extracted from two cultures of tobacco crown gall tumor tissue: an unorganized tissue and a teratoma which produced leafy shoots. On Sephadex LH-20 column chromatography, extracts of both types of tissue yielded two peaks of cytokinin activity with elution volumes similar to
Acid phosphatase activity of normal and tumor tissue of tobacco grown in vitro and in vivo.
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Tissue cultures of Nicotiana labacum consisting of green, albino and habituated (normal origin) and teratoma (tomorous origin) were grown under asceptic conditions for 6 to 8 weeks and their extracts were analyzed for phosphatase activity. Comparative enzyme analyses were also made on crude stem
Superoxide dismutase and peroxidase are coordinately regulated in differentiated and transformed tissues of Nicotiana tabacum.
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We used a series of normal and Agrobacterium-transformed, bacteria-free tobacco tissue cultures which differ in their levels of histodifferentiation to test the relationship of superoxide dismutase, peroxidase, and catalase to oncogenic transformation and differentiation. When compared with normal
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