[Effects of cholinergic drugs and blood plasma proteins on the development of Arthus reaction].

Delayed-type hypersensitivity (DTH) reaction was induced in (CBA x C57BL/6) F1 mice by subcutaneous injection of complete Freund adjuvant (0.02 ml) at the base of the tail. The effects of methacine (2 mg/kg), ipratropium bromide (0.01 mg/kg), their combinations with neostigmine (0.02 mg/kg), hexamethonium (10 mg/kg), acetylcholine (2 microg/kg), nicotine (0.5 mg/kg), gamma globulin and CRP (both 1 mg/kg), and albumin (500 mg/kg) on DTH reaction development, B cell functions and Arthus reaction were investigated. It was established that ipratropium bromide and CRP prevented Arthus reaction development. The administration of acetylcholine, nicotine, and combinations of muscarinic antagonists with neostigmine, as well as gamma globulin and albumin resulted in the later onset of Arthus reaction. The administration of hexamethonium increased DTH reaction and led to early appearance of the Arthus reaction and its maintenance during 21 days. These results demonstrate the role of cholinergic system and plasma proteins in the organism sensitization development.