Daily consumption of 30 g of raw, fresh, broccoli sprouts, not cooked, during 10 weeks (70 days), followed by other 90 days of no ingestion of broccoli sprouts

Obesity

Inflammation

Broccoli sprouts

Effects of the Consumption of Broccoli Sprouts in Overweight Subjects

Plasma levels of IL-6 measured at days 0, 70, 90 and 160 days of intervention

Plasma levels of C-reactive protein measured at days 0, 70, 90 and 160 days of intervention

24 h-urine levels of glucosinolates and their metabolites measured by UHPLC-MS/MS

Débora Villaño

Proffesor

Universidad Católica San Antonio de Murcia

National Research Council, Spain

The intervention consisted on a 10-week period which included daily consumption of a portion (30 g) of raw, fresh broccoli sprouts. After the intervention period, a follow-up recovery period for all subjects continued for other 90 days with no ingestion of broccoli sprouts.

To measure the variation in extent of ITC excretion in urine from a capsule delivered dose of 100mg glucoraphanin

Healthy

The extent of ITC excretion in urine from a capsule delivered dose of glucoraphanin

100mg glucoraphanin

Institute Of Food Research

The Conversion of ENcapsulated GlucorAphanin, Gut Microbiota Phylogeny and gEnotype Study (ENGAGE)

A Human Intervention Trial Investigating the Conversion of Encapsulated Glucoraphanin to Isothiocyanates; and the Potential Link Between the Extent of the Conversion to an Individual's Gut Microbiota Phylogeny and Genotype.

To measure urinary ITC by HP-LC and LC-MS using validated methods in urine collected during a dietary restriction period both before and after (24 hour urine collection) consumption of a 100mg glucoraphanin capsule.

To measure gut microbiota populations by faecal bacteria phylogenetic analysis.

To determine the genotype of participants for key polymorphic genes by PCR on DNA extracted from blood.

Quadram Institute Bioscience

Biotechnology and Biological Sciences Research Council

The Danish Council for Strategic Research

A healthy Nordic diet with high content of bitter strong tasting vegetables and cabbages.

A diet habitually consumed in the Nordic countries

A healthy Nordic diet with high content of sweet and mild tasting vegetables and cabbages.

Type 2 Diabetes

The participants in the two experimental arms will each have to consume 500 g of vegetables and cabbages daily.

Diet intervention

Click here for more information about this study: Maximising the Taste and Health Value of Plant Food Products (MAXVEG)

Hospital Vendsyssel, Center for Clinical Research

Maximising the Taste and Health Value of Plant Food Products

Maximising the Taste and Health Value of Plant Food Products - Impact on Vegetable Consumption, Consumer Preferences and Human Health Factors.

Measured as the AUC from an Oral Glucose Tolerance Test

The fasting blood samples will be analyzed for: glucose, insulin, glucagon, HbA1c, lipids (triglycerides, HDL-, LDL- and total cholesterol), cytokines (, adipokines biomarkers of oxidative stress parathyroid hormone, vitamin D and GLP-1.
Urine samples for metabolomics (isoprostanes and water-soluble metabolites).

Per Bendix Jeppesen

Associate prof., PhD

Aarhus University Hospital

The broccoli sprouts and water are chopped in a blender until a uniform mix is obtained. Salt or sugar may be added to the "shake".

Alfalfa sprouts and water are chopped in a blender until a uniform mix is obtained. Salt or sugar may be added to the "shake".

Smoking

Subjects will drink the broccoli shake homogenate

Broccoli sprout homogenate

Subjects will drink the alfalfa sprout homogenate.

Alfalfa sprout homogenate

UNC Center for Environmental Medicine, Asthma and Lung Biology

Effects of Sulforaphane (SFN) on Immune Response to Live Attenuated Influenza Virus in Smokers and Nonsmokers

Effects of Sulforaphane (SFN)on Innate Immune Responses to Live Attenuated Influenza Virus in Smokers and Nonsmokers

This will be performed by repetitive spraying of sterile normal saline irrigation solution (5 ml total) into the nostril, followed by voluntary expelling of fluid by the subject into a specimen collection cup. Both nostrils are lavaged in this way and the resulting nasal lavage fluid (NLF) from both sides is combined.

Nasal lavage is as previously described. Blood collection will be via venipuncture and nasal epithelial cells will be collected during a nasal scrape.

brassica nigra/anti inflammatoire

Effects of the Consumption of Broccoli Sprouts in Overweight Subjects

The Conversion of ENcapsulated GlucorAphanin, Gut Microbiota Phylogeny and gEnotype Study (ENGAGE)

Maximising the Taste and Health Value of Plant Food Products

Effects of Sulforaphane (SFN) on Immune Response to Live Attenuated Influenza Virus in Smokers and Nonsmokers

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