A detoxified recombinant E. coli heat-labile enterotoxin mutant, LTS61K, is employed as a carrier protein to conjugate polysaccharide. The LTS61K contains a mutated mature sub-unit A (LTA) that includes lysine at amino acid position 61 and a wild-type mature sub-unit B (LTB). Various types of bacterial capsular polysaccharide antigens were chemically conjugated with the LTS61K protein by a reductive amination reaction. The conjugated polysaccharide-LTS61K products were physically, chemically and biochemically identified as soluble form. Rabbits were immunized intramuscularly to determine the immunogenicity of conjugated vaccines by ELISA to detect anti-polysaccharide antigen IgG titers and serum bactericidal assay thereby determining the functional activity of the antibodies. Study results show that conjugated polysaccharide-LTS61K vaccines induce higher polysaccharide-specific IgG titers and greater bactericidal activity in sera than that of polysaccharide alone or polysaccharide mixed with LTS61K. The presence of anti-LTS61K serum IgG antibody alleviates travel diarrhea caused by E. coli (ETEC enterotoxigenic E. coli).

Hsu; Yu-Shen

Kou; I-Ling

Hung; Kuo-Chan

Lu; Yuan-Hsin

Yuan; Ta-Tung

DEVELOPMENT CENTER FOR BIOTECHNOLOGY

Polysaccharide conjugation with detoxified E. coli heat labile enterotoxin (LT) used as vaccine

The present invention relates to microcapsules and to methods for them. The microcapsules comprise a polymeric coating that is at least partly crosslinked with a polymeric matrix comprising a polysaccharide. In the matrix, a lipid and an active agent or a prodrug or salt thereof are embedded, the lipid preferably having a melting point of at least 30.degree. C. The microcapsules can be used as a medicament, dietary supplement or food additive, the microcapsules comprising a polymeric coating that is at least partly crosslinked with a polymeric matrix comprising a polysaccharide. As above, a lipid and an active agent or a prodrug or salt thereof are embedded in the matrix, with the lipid preferably having a melting point of at least 30.degree. C. Methods for treating diarrhea or preventing colon cancer comprising administering such microcapsules are also disclosed.

FRESENIUS KABI DEUTSCHLAND GMBH

Microcapsules with polymeric coating comprising a lipid and an active agent

A method for treating diarrhea in children. The method boils in water a natural source of complex carbohydrates and processes the resulting aqueous solution of complex carbohydrates to produce an aqueous solution of dextrorotatory polysaccharides having a desired osmolarity and electrolyte concentration.

polysaccharide/biegunka

Polysaccharide conjugation with detoxified E. coli heat labile enterotoxin (LT) used as vaccine

Microcapsules with polymeric coating comprising a lipid and an active agent

Amylase-electrolyte oral rehydration method and composition

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