Nowadays, the potential therapeutic role of various bioflavonoids includingCurcumin, Luteolin and Resveratrol has currently been well-documented in a vast range of fatal complicationsincluding synaptic failure and cancers. These bioflavonoids are widely being implementedfor the treatment of various cancers as they possess anti-cancerous, anti-oxidant and anti-inflammatoryproperties. Moreover, they are also used as a better alternative to conventional therapies since; theseare non-toxic to cells and having no or least side effects. Notably, the pertinent therapeutic role of Rutinin cervical cancer is still unsettled however, its anti-cancerous role has already been reported inother cancers including prostate and colon cancer. Rutin (Vitamin P or Rutoside) is a polyphenolicsflavonoid exhibiting multi-beneficial roles against several carcinomas.Despite the evidence for its several biological activities, the anticancer effects of Rutin onhuman cervical cancer (C33A) cells remain to be explored. In this study, the anticancer potential ofRutin was investigated by employing the key biomarkers such as nuclear condensation reactive oxygenspecies (ROS), apoptosis, and changes in mitochondrial membrane potential (MMP).Our findings showed that Rutin treatment reduced the cell viability, induced significant increasein ROS production and nuclear condensation in dose-dependent manner. Moreover, Rutin provokedapoptosis by inducing decrease in MMP and activation of caspase-3. Cell cycle analysis furtherconfirmed the efficacy of Rutin by showing cell cycle arrest at G0/G1 phase.Thus, our study is envisaged to open up interests for elucidating Rutin as an anticancerousagent against cervical cancer.