[Cloning,Analysis and Expression on Gene of Squalene Synthase in Citrus reticulata Seeds].

To clone squalene synthase gene( ss) from seeds of Citrus reticulate ‘Dahongpao’,and to analyze its expression and bioinformatics.

The technology of RT-PCR was used to clone the gene of Citrus reticulata seeds and analyze its expression at various growing stages. Moreover, the bioinformatics was analyzed by online analytical tools.

The full-length of ss was1 205 bp and 397 amino acids were encoded, SS had a homology of 99% with that of Citrus sinensis and Citrus clementina. The growth process of Citrus reticulata seeds was divided into three stages: morphogenesis stage, maturity and dehydration periods, and expression of ss was the highest in maturity period.

Squalene synthase gene from seeds of Citrus reticulate ‘Dahongpa’is obtained for the first time, which provides reference for biosynthesis and regulation mechanism research of limonin compounds in Citrus reticulata seeds.