Decrease in alkaline triglyceride lipase in primary cultured hepatocytes from mice with sarcoma 180.
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Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions of (3H)labeled trichloracetic acid-insoluble materials by hepatocytes from both normal and tumor-bearing mice increased similarly. However, the alkaline triglyceride lipase activity of a homogenate of freshly isolated hepatocytes from tumor-bearing mice was one-third that of cells from normal mice. The activity of hepatocytes from tumor-bearing mice increased less during culture than did the activity of cells isolated from normal mice.