Chinese Journal of Oncology 2003-Sep
[Hepatitis B x protein activated vascular endothelial growth factor expression through hypoxia inducible factor-1 pathway].
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OBJECTIVE
To investigate whether hepatitis B x protein (HBx) stimulates vascular endothelial growth factor (VEGF) through hypoxia inducible factor-1 (HIF-1 alpha) pathway.
METHODS
Two plasmids including pIRES-EGFP-HBx and pTK-Hyg were co-transfected to a hepatocellular carcinoma cell line SMMC-7721. With fluorescence-positive and fluorescence-negative hygromycin-resistant colonies selected, expressions of VEGF and HIF-1 alpha in protein or/and mRNA level were detected.
RESULTS
Fluorescence-positive cells were stably integrated with HBx, in which expression of HIF-1 alpha and VEGF were upregulated. Fluorescence-negative cells did not express HBx, VEGF or HIF-1 alpha.
CONCLUSIONS
HBx can activate VEGF through HIF-1 alpha pathway.
