Nanoliposomes Encapsulation of Enriched-Phenolic Fraction from Pistachio Hulls and It's Antioxidant, Anti-Inflammatory and Anti-Melanogenic Activities.
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Owhadi is a popular commercial pistachio cultivar in Iran which could be an attractive source for natural bioactive compounds with health-promoting activity.The hulls subjected to fractionation and ethyl acetate fraction was a phenolic-enriched fraction (PEF). The PEF was encapsulated in nanoliposomes (PEF-NLs) as a newly developed delivery system. The phytochemical analysis of PEF-NLs confirmed the presence of phenolic and flavonoid compounds.The PEF-NLs indicated the strong antioxidant activity through up-regulation of the antioxidant-related genes in the murine hepatocyte. The PEF-NLs indicated the notable anti-inflammatory activity by scavenging the nitric oxide (NO) and reducing the NO production in the murine macrophage cells. The PEF-NLs has also exhibited the anti-melanogenic activity through direct tyrosinase enzyme inhibition and by modulating of melanin biosynthesis genes in B16F10 melanoma cells.The PEF-NLs possessed the promising potential to be used for controlling skin pigmentation disorders and as a skin-whitening agent in the cosmetic industry.
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