Journal of Applied Microbiology 2020-Feb
Antifungal Activity of Cinnamaldehyde Against Fusarium sambucinum Involves Inhibition of Ergosterol Biosynthesis.
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METHODS AND RESULTS
Minimum inhibitory concentration and minimal fungicidal concentration of cinnamaldehyde were 3 and 4 mmol l-1 on spore germination and colony development assays in vitro, respectively. Furthermore, the lesion diameter of potato tubers and tuber slices inoculated with F. sambucinum was reduced by 76.9% and 69% after treatment with 4 mmol l-1 cinnamaldehyde. Cytometric analyses reveled that cinnamaldehyde significantly affected the integrity of cell membrane firstly, then decreased mitochondrial membrane potential and induced the accumulation of intracellular reactive oxygen species. Meanwhile, High performance liquid chromatography (HPLC) results indicated that 3 mmol l-1 cinnamaldehyde could reduce the ergosterol content by 67.94%. This effect was accompanied by a down regulation of ERG11, ERG6 and ERG4 which were involved in ergosterol biosynthesis.