Hovenia dulcis polysaccharides: Influence of multi-frequency ultrasonic extraction on structure, functional properties, and biological activities.

The directional effect of single-frequency ultrasonic was the cause of the low extraction yield of polysaccharide macromolecule. Thus, a possible solution was to use multi-frequency ultrasonic technology to improve the yield of polysaccharide. Single-frequency (SF), dual-frequency (DF), and three-frequency (TF) ultrasonic extraction were applied to extract polysaccharides of Hovenia dulcis (HDPs). A maximal polysaccharide extraction yield (9.02 ± 0.29%) was gat using the dual-frequency ultrasonic with optimized DF conditions comprising 58.00 °C, 33.00 min, 28&40 kHz. The three HDPs were compared for their physicochemical, rheological, and functional properties, and their antioxidant activities. DF-HDPs contain higher uronic acid than SF-HDPs and TF-HDPs. Rheological tests indicated that the HDPs had excellent colloid properties and a promising potential to serve as a thickener, gelatinizer, and stabilizing agent in the food industry. Moreover, the DF-HDPs exhibited a notable oil holding capacity (3.92 ± 0.04 g oil/g), foaming capacity (35.26 ± 0.47%), and emulsion capacity (43.96 ± 0.67%). Compared to the SF- and TF-HDPs, the DF-HDPs had superior antioxidant activities. In conclusion, a better extraction method (dual-frequency ultrasonic extraction) was achieved.