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Molecular mechanisms underlying he interaction of motuporin and microcystins with type-1 and type-2A protein phosphatases.

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Heptapeptide microcystin and pentapeptide motuporin (nodularin-V) are equipotent inhibitors of type-1 and type-2A protein phosphatase catalytic subunits (PP-1c and PP-2Ac). Herein we describe elucidation of the molecular mechanisms involved in the interaction of these structurally similar

Interaction of procion red HE-3B and other reactive dyes with alkaline phosphatase: a study by means of kinetic, difference spectroscopic and chromatographic methods.

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The interaction of alkaline phosphatase (EC 3.1.3.1) from calf intestine with different dyes, especially with Procion Red HE-3B was studied by several methods. From the kinetic analysis a nonlinear noncompetitive type of inhibition with an inhibition constant Ki = 0.03 mM for Procion Red HE-3B and

Enzyme and immunohistochemical phenotyping of diethylnitrosamine-induced liver lesions of male C3H/He, B6C3F1 and C57BL/6J mice.

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Male C3H/He, B6C3F1 and C57BL/6J mice were given a single injection of diethylnitrosamine (20 micrograms/g body wt) on day 15 after birth and animals were killed 17-29 (C3H/He and B6C3F1) and 29-46 weeks (C57BL/6J) after treatment. Carcinogen-induced liver lesions were identified by a deficiency in

Effect of low-energy laser (He-Ne) irradiation on the process of bone repair in the rat tibia.

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The effect of low-energy laser (He-Ne) irradiation on bone repair in the tibia of the rat after hole injury was investigated using biochemical and quantitative histomorphometrical methods. The activity of alkaline phosphatase (ALP) showed a sharp peak at 6 days post-injury and a lower peak at 12

Multienzyme control serum (Seraclear-HE) containing human enzymes from established cell lines and other sources. 1: Preparation and properties.

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We have developed a new multienzyme control serum, Seraclear-HE, which was designed to function not only as an accuracy and precision control serum but also as an intermethod calibrator for unifying interlaboratory clinical enzyme data in terms of reference method values. Seraclear-HE contains as

Promotion of bone repair in the cortical bone of the tibia in rats by low energy laser (He-Ne) irradiation.

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The effect of low energy laser (He-Ne) irradiation on bone repair in the cortical part of the tibia of the rat was investigated using biochemical and radioactive labeling methods. A fixed round hole was created in the lateral aspect of the tibia and the newly formed tissue was collected from the gap

Role of mutations at codon 61 of the c-Ha-ras gene during diethylnitrosamine-induced hepatocarcinogenesis in C3H/He mice.

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Liver tumors of certain strains of mice frequently contain mutations at codon 61 of the c-Ha-ras gene. In our study, we investigated the significance of these mutations in the carcinogenic process. Male C3H/He mice received a single injection of diethylnitrosamine (DEN) on day 15 after birth, and

Diagnostic and prognostic values of critical flicker frequency determination as new diagnostic tool for objective HE evaluation in patients undergoing TIPS implantation.

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OBJECTIVE The diagnostic and prognostic value of critical flicker frequency (CFF) analysis for assessment of severity and dynamics of hepatic encephalopathy (HE) was studied before and after implantation of a transjugular intrahepatic portosystemic shunt (TIPS). METHODS Sixty-three cirrhotic

Effects of treatment of the fat body trophocytes of Melipona quadrifasciata anthidioides nurse workers and virgin queens in culture by juvenile hormone III and ecdysterone (20-HE).

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The fat body (FB) consists of two types of cells: throphocytes and oenocytes. Throphocytes are related to intermediary metabolism storing lipids, carbohydrates, and proteins while oenocytes play role in the lipids and lipoproteins production. The vitellogenin is the precursor of egg yolk (vitelline)

Ecdysteroid mediated fat body acid phosphatase activity during larval development of rice moth, Corcyra cephalonica (Lepidoptera).

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20-hydroxyecdysone (20-HE) stimulates acid phosphatase activity in the fat body of ligated late-last instar larvae. This effect is time dependent and the specific activity of enzyme increases significantly in hormone treated insects. 20-HE also stimulates general protein synthesis. Cycloheximide

Shigeru Tsuiki: a pioneer in the research fields of complex carbohydrates and protein phosphatases.

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Dr Tsuiki made three major contributions during his illustrious career as a biochemist. First, he developed the procedure for mucin isolation from bovine submaxillary glands. His work became the basis for mucin biochemistry. Second, he identified four distinct molecular species of mammalian

[The effect of low-intensity radiation from a helium-neon laser on the alkaline phosphatase activity in an uncomplicated mandibular fracture and in traumatic osteomyelitis].

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The osteal alkaline phosphatase (AP) activity was examined in 167 Wistar rats with uncomplicated regeneration and traumatic osteomyelitis of the mandible, exposed to low-intensity He-Ne laser. Laser exposure stimulated the bone AP activity in the animals with traumatic osteomyelitis and

Extremely elevated serum alkaline phosphatase level upon treatment with teriparatide: a case report

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Background: Teriparatide is a homolog of human parathyroid hormone (1-34), which is approved for the treatment of postmenopausal and glucocorticoid-induced osteoporosis. Several minor and transient side effects have been reported for

[A quantitative histochemical study of alkaline phosphatase isoenzyme kinetics in the thyroid gland, kidney and small intestine of white rats].

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Microphotometric determination of alkaline phosphatase isoenzyme patterns in morphological structures of rat thyroid gland, kidney and small intestine were performed, using different concentration of L-levamisol and temperature-dependent enzyme inactivation. It was concluded that he constant of

Age- and diapause-related acid and alkaline phosphatase activities in the intestine and malpighian tubules of the Colorado potato beetle, Leptinotarsa decemlineata (Say).

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Specific activities for soluble (s) and membrane (m)-bound acid (ACP) and alkaline phosphatases (ALP) were determined in the midgut, hindgut, and Malpighian tubules for developing, prediapausing, and diapausing adult Colorado potato beetles, Leptinotarsa decemlineata (Say). High ACP activities were

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