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asparagine/milho

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Metabolic regulation of asparagine synthetase gene expression in maize (Zea mays L.) root tips.

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Differential hybridization of a cDNA library constructed with poly(A)+ mRNA from 24 h starved maize (Zea mays L.) root tips, resulted in the isolation of a cDNA (called pZSS1) that was highly induced during glucose deprivation. The nucleotide sequence analysis of the full-length cDNA allowed its
Glutamine synthetase (GS) and asparagine synthetase (AS) are proposed to have important function in plant nitrogen (N) remobilization, but their roles under drought stress are not well defined. In this study, the expression dynamics of GS and AS genes were analyzed in two maize varieties (ZD958 and

Asparagine synthesis in Zea mays.

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Regulation of Nitrate Reductase during Early Seedling Growth (A Role for Asparagine and Glutamine).

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Growth systems that either permit (wet system) or prevent (dry system) the hydrolysis of endosperm reserves in maize (Zea mays) seedlings were developed to study the effect of endosperm reserves on the acquisition of external nitrogen. Three-day-old seedlings treated with 5 mM KNO3 for 24 h had

Asparagine metabolism and nitrogen distribution during protein degradation in sugar-starved maize root tips.

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Excised maize (Zea mays L.) root tips were used to monitor the effects of prolonged glucose starvation on nitrogen metabolism. Following root-tip excision, sugar content was rapidly exhausted, and protein content declined to 40 and 8% of its initial value after 96 and 192 h, respectively. During
The cut ends of excised Zea mays roots were sealed to a pressure transducer and their root pressures recorded. These rose approximately hyperbolically to a maximum value of 4.21 +/- 0.34 bar after 30 to 40 minutes. Xylem exudate could not be collected at this pressure since the flow rate was zero.

Free asparagine and sugars profile of cereal species: the potential of cereals for acrylamide formation in foods.

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Cereals-based food is one of the major source of Maillard reaction products in the diet. Free amino acids and reducing sugars are considered to be the main precursors in the formation of these heat-induced compounds. In order to determine genetic resources with reduced potential for acrylamide
*The main steps of nitrogen (N) metabolism were characterized in the developing ear of the two maize (Zea mays) lines F2 and Io, which were previously used to investigate the genetic basis of nitrogen use efficiency (NUE) in relation to yield. *During the grain-filling period, we monitored changes

Asparagine synthetase in corn roots.

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The level of asparagine synthetase is low in 10-mm root tips from corn seedings (Zea mays W64 x W182F) but relatively high in mature root sections taken 20 to 35 mm from the tip. When root tips are excised there is a marked increase in asparagine synthetase over a 5-hour period. In mature root

Enzymes of asparagine synthesis in maize roots.

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An asparagine synthetase which is active with either glutamine or NH 4 (+) has been found in maize (Zea mays L.) roots. Unlike the enzyme obtained from legume cotyledons, the maize-root enzyme is only slightly more efficient with glutamine (Km, 1.0 mM) than with NH 4 (+) (Km, 2.0-3.0 mM). The
Three-week-old maize (Zea mays L.) plants were submitted to light/dark cycles and to prolonged darkness to investigate the occurrence of sugar-limitation effects in different parts of the whole plant. Soluble sugars fluctuated with light/dark cycles and dropped sharply during extended darkness.
Drought stress during reproductive growth stages greatly affects the growth and productivity of maize plants. To better understand the metabolic regulation during post-silking drought (PD) stress, an RNA sequencing (RNA-Seq) analysis was performed at the late stage of leaf senescence in maize.
A detailed network describing asparagine metabolism in plants was constructed using published data from Arabidopsis (Arabidopsis thaliana) maize (Zea mays), wheat (Triticum aestivum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupus albus), and other species, including animals. Asparagine

Application of NMR-based metabolomics to the investigation of salt stress in maize (Zea mays).

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BACKGROUND High salinity, caused by either natural (e.g. climatic changes) or anthropic factors (e.g. agriculture), is a widespread environmental stressor that can affect development and growth of salt-sensitive plants, leading to water deficit, the inhibition of intake of essential ions and

Kinetics of NH(4) Assimilation in Zea mays: Preliminary Studies with a Glutamate Dehydrogenase (GDH1) Null Mutant.

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In higher plants it is now generally considered that glutamate dehydrogenase (GDH) plays only a small or negligible role in ammonia assimilation. To test this specific point, comparative studies of (15)NH(4) (+) assimilation were undertaken with a GDH1-null mutant of Zea mays and a related (but not
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