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Identification of a unique 2-oxoglutarate-dependent flavone 7-O-demethylase completes the elucidation of the lipophilic flavone network in basil.

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Small molecule demethylation is considered unusual in plants. Of the studied instances, the N-demethylation of nicotine is catalyzed by a Cyt P450 monooxygenase, while the O-dealkylation of alkaloids in Papaver somniferum is mediated by 2-oxoglutarate-dependent dioxygenases (2-ODDs). This report

Molecular cloning and functional characterization of an antifungal PR-5 protein from Ocimum basilicum.

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Pathogenesis-related (PR) proteins are involved in biotic and abiotic stress responses of plants and are grouped into 17 families (PR-1 to PR-17). PR-5 family includes proteins related to thaumatin and osmotin, with several members possessing antimicrobial properties. In this study, a PR-5 gene

First Report of Alfalfa mosaic virus Infecting Basil (Ocimum basilicum) in California.

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Basil (Ocimum basilicum L.) plants collected from three fields in Imperial County, CA in May, 2011 were found to be exhibiting yellowing, chlorotic sectors and spots on leaves, resulting in unmarketable plants. Dodder (Cuscuta spp.) was present in one of the fields, but was not visibly associated

First Report of Basil Leaf Spot Caused by Pseudomonas cichorii in Louisiana and Cultivar Screening Results.

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A leaf spot of basil, Ocimum basilicum L., was observed on container-grown and field plantings of cultivars Aussie Sweet and Sweet Basil. The disease was of minor importance under field conditions, but was of potential economic importance in seedling production. Gray to black, watersoaked, necrotic

First Report of Black Stem Caused by Botryosporium longibrachiatum on Sweet Basil in Korea.

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Sweet basil, Ocimum basilicum L., is cultivated mainly for fresh consumption in Korea. In March 2009, in Icheon, Korea, several dozen plants showing symptoms of black stems were found in an organic farm that used polyethylene tunnels for production. The black stems were usually covered with a fungus

First Report of Bacterial Leaf Spot of Basil Caused by Pseudomonas viridiflava in Hungary.

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In April 2011, typical bacterial spot symptoms were observed on sweet basil plantlets (Ocimum basilicum L.) in a supermarket in Budapest, Hungary. Affected plants had dark brown-to-black lesions on the cotyledons. Spots on the leaves were first water soaked and then became necrotic and progressed

First Report of Bacterial Leaf Spot of Basil Caused by Pseudomonas viridiflava in Argentina.

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In June 1998, during a cool, humid period, typical bacterial spot symptoms were observed on basil plantlets (Ocimun basilicum L. 'Royal Louis' and 'Zaes') in a commercial greenhouse in La Plata, Argentina. Affected plants had dark brown to black lesions on cotyledons. Spots on leaves were first

First Report on Natural Occurrence of Tomato Spotted Wilt Tospovirus in Basil (Ocimum basilicum).

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Virus-like symptoms were observed on basil plants (Ocimum basilicum L. 'Mrs. Burns Lemon' [MBL]) growing in containers and a demonstration plot at the Louisiana State University Burden Research Plantation, Baton Rouge, during July 1998. Symptoms consisted of ring spots, leaf distortion, and severe

First Report of Tobacco vein banding mosaic virus in China (Xian, Shaanxi Province) in Datura stramonium and Tobacco.

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Tobacco vein banding mosaic virus (TVBMV) has been reported in Taiwan (1), North America (Tennessee) (2), and Japan (3) and induces a severe disease of tobacco. During surveys on viruses of vegetables in China, TVBMV was isolated from a Datura stramonium weed plant in July 1998 in Shaanxi Province.

First Report of Impatiens necrotic spot virus (INSV) Infecting Basil (Ocimum basilicum) in the United States.

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Basil (Ocimum basilicum L.), a native of India belonging to the Lamiaceae family, is an aromatic herb with distinctive aroma, and several commercial varieties are used extensively for culinary and ornamental purposes. During the summer of 2011 and 2012, potted plants of basil in a commercial

Uptake of nicotine from discarded cigarette butts - A so far unconsidered path of contamination of plant-derived commodities.

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This study aimed to elucidate the origin of the widespread nicotine contamination of plant-derived commodities, by conducting field experiments with various herbs and spice plants. By scattering tobacco and cigarette butts on the field and subsequent nicotine analyses of the acceptor plants, we

Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems.

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Numerous aromatic plant species produce high levels of monoterpenols, using geranyl diphosphate (GPP) as a precursor. Sweet basil (Ocimum basilicum) geraniol synthase (GES) was used to evaluate the monoterpenol profiles arising from heterologous expressions in various plant models. Grapevine (Vitis

A thaumatin-like protein of Ocimum basilicum confers tolerance to fungal pathogen and abiotic stress in transgenic Arabidopsis.

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Plant often responds to fungal pathogens by expressing a group of proteins known as pathogenesis-related proteins (PRs). The expression of PR is mediated through pathogen-induced signal-transduction pathways that are fine-tuned by phytohormones such as methyl jasmonate (MeJA). Here, we report

Virus-Induced Gene Silencing in Sweet Basil (Ocimum basilicum)

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Virus-induced gene silencing (VIGS) is a powerful reverse genetic tool for rapid functional analysis of plant genes. Over the last decade, VIGS has been widely used for conducting rapid gene knockdown experiment in plants and played a crucial role in advancing applied and basic research in plant

Isolation and functional characterization of a τ-cadinol synthase, a new sesquiterpene synthase from Lavandula angustifolia.

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In this paper we characterize three sTPSs: a germacrene D (LaGERDS), a (E)-β-caryophyllene (LaCARS) and a τ-cadinol synthase (LaCADS). τ-cadinol synthase is reported here for the first time and its activity was studied in several biological models including transiently or stably transformed tobacco

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