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Analysis of exposed cellulose surfaces in pretreated wood biomass using carbohydrate-binding module (CBM)-cyan fluorescent protein (CFP).

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In enzymatic saccharification of lignocellulosics, the access of the enzymes to exposed cellulose surfaces is a key initial step in triggering hydrolysis. However, knowledge of the structure-hydrolyzability relationship of the pretreated biomass is still limited. Here we used fluorescent-labeled

Effect of forest land use change on carbohydrates, physical soil quality and carbon stocks in Moroccan cedar area.

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The present work was conducted to evaluate the carbon storage potential of Moroccan cedar forest and its vulnerability to transformations through deforestation and overgrazing. The present study took place in the Middle Atlas where is the most beautiful of Moroccan cedar forest (Cedrus atlantica).

Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses.

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BACKGROUND Carbohydrates expressed on allergens are known to be important for allergenicity. However, little is known about whether the carbohydrates drive the T(H)2 response. OBJECTIVE We sought to determine a role for carbohydrates expressed on Cry j 1, which is the major allergen of Cryptomeria

NMR Analysis on Molecular Interaction of Lignin with Amino Acid Residues of Carbohydrate-Binding Module from Trichoderma reesei Cel7A.

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Lignocellulosic biomass is anticipated to serve as a platform for green chemicals and fuels. Nonproductive binding of lignin to cellulolytic enzymes should be avoided for conversion of lignocellulose through enzymatic saccharification. Although carbohydrate-binding modules (CBMs) of cellulolytic

Carbohydrate structures of the glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen.

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The glycoprotein allergen Cry j I from Japanese cedar (Cryptomeria japonica) pollen was treated with pepsin and glycopeptidase A to release asparagine-linked oligosaccharides. The reducing ends of the oligosaccharides were aminated with the fluorescent reagent 2-aminopyridine. The oligosaccharide

Hydroethanolic extract of the inner stem bark of Cedrela odorata has low toxicity and reduces hyperglycemia induced by an overload of sucrose and glucose.

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BACKGROUND Cedrela odorata L. (Meliaceae) is a native plant of the Amazon region and its inner stem bark is used in the treatment of diabetes in the form of maceration in Brazilian popular medicine. Until now, there is no scientific study on this activity. The present study was aimed at evaluating

Interaction of nutrient limitation and elevated CO2 concentration on carbon assimilation of a tropical tree seedling (Cedrela odorata).

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Carbon assimilation by Cedrela odorata L. (Meliaceae) seedlings was investigated in ambient and elevated CO2 concentrations ([CO2]) for 119 days, using small fumigation chambers. A solution containing macro- and micronutrients was supplied at two rates. The 5% rate (high rate) was designed to avoid

Catalytic Performance of Food Additives Alum, Flocculating Agent, Al(SO4)3, AlCl3, and Other Lewis Acids in Microwave Solvolysis of Hardwoods and Recalcitrant Softwood for Biorefinery.

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The development of a novel pretreatment system using catalysts with high safety and low cost is pivotal to establish lignocellulosic biorefinery. We evaluated 16 Lewis acid catalysts for microwave solvolysis pretreatment to enhance enzymatic saccharification of woody biomass and found that very

N-terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j I).

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A purified preparation of a major allergen of Japanese cedar pollen, sugi basic protein (SBP, Cry j I), was separated into 5 subfractions of 50-45 kDa. All of the SBP subfractions were confirmed to be reactive to IgE antibodies from patients with Japanese cedar pollinosis, and also to mouse anti-SBP

The natural history of mountain cedar pollinosis.

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During the winter months, pollen from the mountain cedar (MC) (Juniperus sabinoides) causes severe respiratory tract allergy in central Texas. We have been impressed with the fact that many of our MC-allergic patients had only allergic rhinitis and were only sensitive to MC pollen. We therefore

The natural history of mountain cedar pollinosis.

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Antigenicity of the oligosaccharide moiety of the Japanese cedar (Cryptomeria japonica) pollen allergen, Cry jI.

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Cry jI, a major allergenic glycoprotein of Cryptomeria japonica (Japanese cedar, Sugi), is the most common pollen allergen in Japan. Carbohydrate analysis and lectin staining indicated that Cry jI possesses the fucose/xylose-containing N-linked oligosaccharide which previously has been found in some

Molecular cloning of major allergen from Cupressus arizonica pollen: Cup a 1.

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The family Cupressaceae is a relevant source of allergens that causes winter respiratory allergies. Cloning and sequencing the major antigen of Cupressus arizonica is important for a better diagnosis and treatment of sensitized patients. To obtain a full-length complementary DNA for Cup a 1, the

Screening of fungi for decomposition of lignin-derived products from Japanese cedar.

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Lignin is an aromatic polymer that makes a network by intertwining between cellulose fibers in plant. As the lignin network retards access to carbohydrates, it is regarded as a nuisance during biomass processing. When wood is processed into paper pulp or bioethanol, lignin is produced as a

Effects of lignins as diet components on the physiological activities of a lower termite, Coptotermes formosanus Shiraki.

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We investigated the effects of lignins as diet components on the physiological activities of a lower termite, Coptotermes formosanus Shiraki. Artificial diets composed of polysaccharides with and without purified lignins (milled-wood lignins) from Japanese cedar (softwood), Japanese beech

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