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Colchicine-mediated chromosome doubling during anther culture of maize (Zea mays L.).

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Efficient methods of chromosome doubling are critical for the production of microspore-derived, doubled-haploid (=DH) plants, especially if, as in maize anther culture, spontaneous chromosome doubling occurs infrequently. In the present study, colchicine (5-1000 mg/l) was added to the induction

Colchicine antimitosis abolishes CCl4 autoprotection.

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A subtoxic dose of CCl4 is known to destroy liver microsomal cytochrome P-450 and this is widely accepted as the mechanism of CCl4 autoprotection. Circumstantial evidence suggests that while cytochrome P-450 is significantly decreased, this mechanism alone cannot explain the phenomenon of

The control of cellulose microfibril deposition in the cell wall of higher plants : II. Freeze-fracture microfibril patterns in maize seedling tissues following experimental alteration with colchicine and ethylene.

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Cells of maize (Zea mays L.) seedling that are not fixed or cryoprotected contain the impressions of cellulose microfibrils on freeze-fractured plasma membranes. Impressions of the most recently deposited microfibrils have terminal complexes associated with them (see preceding paper). The

Efficient production of doubled haploid plants through colchicine treatment of anther-derived maize callus.

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A chromosome doubling technique, involving colchicine treatment of an embryogenic, haploid callus line of maize (Zea mays L., derived through anther culture), was evaluated. Two colchicine levels (0.025% and 0.05%) and three treatment durations (24, 48, and 72 h) were used and compared to untreated

Effect of antimitotic agent colchicine on carbon tetrachloride toxicity.

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A single administration of a subtoxic dose of CCl4 (100 microliters/kg, i.p.) is known to induce hepatocellular regeneration and tissue repair at 6 and 48 h in rats, permitting prompt recovery from the limited liver injury associated with that dose of CCl4. Substantial evidence has accumulated to

The effects of grape seed and colchicine on carbon tetrachloride induced hepatic damage in rats.

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This study aims to determine the effects of grape seed and colchicine on carbon tetrachloride (CCl4) induced hepatic damage and on some serum biochemical parameters. Sixty male Wistar albino rats (200-250 g) were randomly divided into six groups (ten rats/group) and included the control group the

Colchicine antimitosis abolishes resiliency of postnatally developing rats to chlordecone-amplified carbon tetrachloride hepatotoxicity and lethality.

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We have previously reported that rats are resilient to the hepatotoxic and lethal combination of chlordecone (CD) and carbon tetrachloride (CCl4) during early postnatal development. The overall findings pointed to stimulated cell division and tissue repair mechanisms as the underlying cause of

Cytogenetic studies in the genus Zea : 2. Colchicine-induced multivalents.

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Premeiotic colchicine treatment brings about the production of one to five quadrivalents in Zea mays ssp. mays (maize, 2n=20) and an increase in the number of quadrivalents from five to ten in Zea perennis (2n=40). The results confirm the allotetraploid nature of maize and suggest that the species

Effect of colchicine on hepatobiliary function in CCl4 treated rats.

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A number of toxic chemicals affect the biliary excretory function of liver. Organochlorines and halomethanes are known to enhance bile flow. Despite the demonstration that a diversity of agents modify biliary function, the mechanism by which these chemicals manifest this effect is not fully

Blockage of mitosis in maize root tips using colchicine-alternatives.

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Mitosis in plants can be blocked by colchicine which has the capacity to bind microtubule subunits. In maize (Zea mays L.) breeding, it is frequently being used for doubling chromosome numbers of haploids for producing homozygous doubled haploids. However, colchicine is highly toxic for mammals and

Cell synchronization and isolation of metaphase chromosomes from maize (Zea mays L.) root tips for flow cytometric analysis and sorting.

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Accumulation of cells containing metaphase chromosomes is an important step in cytological analyses and chromosome sorting procedures. The goal of this research was to optimize treatment parameters to synchronize the cell cycle of maize root tip meristem cells. Levels of hydroxyurea, a DNA synthesis

Meiotic pairing in the hybrid (Zea diploperennis x Zea perennis) x Zea mays and its reciprocal.

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Genomic formulae, fertility, chromosome pairing, and the cryptic intergenomic pairing (induced by using diluted colchicine solution) were analysed in the tri-hybrid (MDP), obtained by crossing DP40 (2n=40, which was inferred in previous studies to have originated from the fusion of an unreduced

QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.).

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A major QTL for SHGD was identified on chromosome 5 with stable expression across environments. The introgression this QTL can overcome the need of colchicine in DH lines development. Genome doubling of haploids is one of the major constraints of large-scale doubled haploid (DH) technology.

Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays).

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Four Japanese wheat varieties, three crossable and one non-crossable with Hordeum bulbosum, were pollinated with maize pollen of 5 genotypes. By the application of 2,4-dichlorophenoxyacetic acid after pollination, embryos kept developing on wheat plants until 14 days after pollination. The frequency

Dietary factors affecting the proliferation of epithelial cells in the mouse colon.

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Corn oil (CO), given as a bolus to female C57BL/6J mice, caused a marked increase in cell proliferation in the colon; this was assessed with a two-hour colchicine arrest for mitotic figures (MF) and with [3H]thymidine incorporation for labeled cells (LC) per crypt measured in histological

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