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Página 1 a partir de 6656 resultados
Proteases: common culprits in human skin disorders.
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Recent findings from the clinic and the laboratory have transformed the way proteases and their inhibitors are perceived in the outermost layer of the skin, the epidermis. It now appears that an integrated proteolytic network operates within the epidermis, comprising more than 30 enzymes that carry
Novel proteases: common themes and surprising features.
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Proteases perform a wide variety of functions, inside and outside cells, regulating many biological processes. Recent years have witnessed a number of significant advances in the structural biology of proteases, including aspects of intracellular protein and peptide degradation by
Common themes and variations in serine protease autotransporters.
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The serine protease autotransporters of the Enterobacteriaceae (SPATEs) represent a group of large-sized, multi-domain exoproteins found only in pathogenic enteric bacteria. These proteins contain a highly conserved channel-forming C-terminal domain, which functions together with YaeT/Omp85 to
Common ancestor of serine proteases and flavin-binding domains.
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Common feature of the four types of protease mechanism.
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Common HIV drug-drug interactions.
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Patients with HIV receive a multitude of medications daily, typically including a triple-drug antiretroviral regimen, prophylactic agents, and therapeutic drugs as needed. While the extensive drug regimens have resulted in improved survival and fewer opportunistic infections, they are also
Individual and common inhibitors of coronavirus and picornavirus main proteases.
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Picornaviruses (PV) and coronaviruses (CoV) are positive-stranded RNA viruses which infect millions of people worldwide each year, resulting in a wide range of clinical outcomes. As reported in this study, using high throughput screening against approximately 6800 small molecules, we have identified
Nitric oxide and the common cold.
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OBJECTIVE
The common cold is a clinical syndrome triggered by a variety of viral pathogens, but rhinoviruses are the most frequent cause. Complications of such infections include sinusitis, otitis media, and exacerbations of asthma and chronic obstructive lung disease. There is growing interest in
Thiol proteases and aldehyde dehydrogenases: evolution from a common thiolesterase precursor?
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The C-terminal 222 residues of human liver aldehyde dehydrogenase can be aligned with the C-terminal 226 residues of a thiol protease from Dictyostelium discoideum to yield 47 residue identities, including matching active site cysteine residues. A multiple alignment with three more aldehyde
Common structural features of IgA1 protease-like outer membrane protein autotransporters.
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Identification of common functional configurations among molecules.
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A new algorithm for identifying three-dimensional configurations of chemical features common to a set of molecules is described. The algorithm scores each configuration based both on the degree to which it is common to the input set and its estimated rarity. The algorithm can be applied to molecules
A novel subtilase from common bean leaves.
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We describe the isolation of a protease from common bean leaves grown in the field. On the basis of its biochemical properties it was classified as serine proteinase belonging to the subtilisin clan. Isoelectric focusing resulted in a single band at pH 4.6, and SDS-PAGE in a single band
Common artifacts and mistakes made in electrophoresis.
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Proteases that act at room temperature upon proteins in the sample buffer prior to heating, cleavage of the Asp-Pro bond upon prolonged heating of proteins at high temperatures, contamination of sample or sample buffer with keratin, leaching of chemicals from disposable plasticware, contamination of
A common pentapeptide conformation occurs in viral acid proteases and other proteins.
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We found a pentapeptide conformation, termed a type I twist, which has a strikingly high propensity (56%) for aspartic acid in the first position. Type I twists include the active site loops from cellular and viral aspartic proteases, with the catalytic Asp in the first position. Fifteen other type
Antigenic determinant common to four kinds of thiol proteases of plant origin.
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