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14 resultados
Preparation of infectious ribonucleic acid from potato virus X by means of guanidine denaturation.
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The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.
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1. Potato lectin has been purified and shown to be a glycoprotein containing about 50% of carbohydrate. Most of the sugar residues (92%) are arabinose; small amounts of galactose, glucose and glucosamine are also present. 2. The most abundant amino acid is hydroxyproline (16% of the residues), 11.5%
The unfolding pathway and conformational stability of potato carboxypeptidase inhibitor.
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The unfolding and denaturation curves of potato carboxypeptidase inhibitor (PCI) were investigated using the technique of disulfide scrambling. In the presence of denaturant and thiol initiator, the native PCI denatures by shuffling its native disulfide bonds and converts to form a mixture of
Effects of guanidine hydrochloride and high pressure on subsite flexibility of beta-amylase.
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We investigated the effects of guanidine hydrochloride (GuHCl) and high pressure on the conformational flexibility of the active site of sweet potato beta-amylase by monitoring the sulfhydryl reaction and the enzymatic activity. The reactivity of Cys345 at the active site, one of six inert half
Starch Flocculation by the Sweet Potato Sour Liquid Is Mediated by the Adhesion of Lactic Acid Bacteria to Starch.
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In the current study, we focused on the mechanism underlying starch flocculation by the sweet potato sour liquid. The traditional microbial techniques and 16S rDNA sequencing revealed that Lactobacillus was dominant flocculating microorganism in sour liquid. In total, 86 bacteria, 20 yeasts, and 10
Actin stimulates plasmin generation by tissue and urokinase-type plasminogen activators.
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The experiments reported here were carried out to define in greater detail actin's stimulation of plasmin generation by t-PA. Actin did not alter t-PA's hydrolysis of a synthetic substrate, and thus is unlikely to have a direct effect upon t-PA's proteolytic activity. When studied in a single-stage
An Internal Reaction Control for Routine Detection of Clavibacter michiganensis subsp. sepedonicus Using a Real-Time TaqMan PCR-Based Assay.
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An internal reaction control was integrated into a TaqMan polymerase chain reaction (PCR) assay for the detection of Clavibacter michiganensis subsp. sepedonicus, the causal organism of bacterial ring rot of potato. The reaction control, cloned into plasmid pCmsC4, consisted of a sequence unrelated
[Isolation of a biologically active poly(A)+RNA from tissues enriched with polysaccharides].
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A special technique is developed for isolation of biologically functional poly(A)+RNA from higher plant cells to start with some procedures used to isolate RNA of the eucaryote cells. The technique includes cell disruption with a 4 M guanidine thiocyanate solution, hot phenol extraction of RNA
Antidiabetic II drug metformin in plants: uptake and translocation to edible parts of cereals, oily seeds, beans, tomato, squash, carrots, and potatoes.
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Residues of pharmaceuticals present in wastewater and sewage sludge are of concern due to their transfer to aquatic and terrestrial food chains and possible adverse effects on nontargeted organisms. In the present work, uptake and translocation of metformin, an antidiabetic II medicine, by edible
Amaranthus caudatus lectin with polyproline II fold: conformational and functional transitions and molecular dynamics.
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Polyproline II (PPII) fold, a peculiar structural element was detected in the Amaranthus caudatus seed lectin (ACL) based on far UV circular dichroism spectrum, conformational transitions of the lectin, and a distinct isodichroic point in thermal denaturation. It was confirmed using PolyprOnline
Antitumor, antioxidant and antimicrobial studies of substituted pyridylguanidines.
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A series of N-pivaloyl-N'-(alkyl/aryl)-N''-pyridylguanidine of general formula C₄H₉CONHC(NR¹R²)NPy have been synthesized and characterized using elemental analysis, FT-IR, multinuclear NMR spectroscopy, and in the case of compounds 7 and 11, by single crystal X-ray diffraction (XRD). The synthesized
Non-leaching antimicrobial biodegradable PBAT films through a facile and novel approach.
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The antimicrobial thermoplastic starch (ATPS) containing guanidine-based polymers was obtained using a twin-screw extrusion with potato starch and polyhexamethylene guanidine hydrochloride (PHGH). Furthermore, the non-leaching antimicrobial biodegradable poly(butylene adipate-co-terephthalate)
Biguanide related compounds in traditional antidiabetic functional foods.
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Biguanides such as metformin are widely used worldwide for the treatment of type-2 diabetes. The identification of guanidine and related compounds in French lilac plant (Galega officinalis L.) led to the development of biguanides. Despite of their plant origin, biguanides have not been reported in
Improved method for the isolation of RNA from plant tissues.
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A fast and efficient method for the isolation of RNA from plant tissues is described. Tuber tissue is homogenized in a guanidine hydrochloride-containing buffer followed by direct extraction with phenol/chloroform. The RNA is precipitated from the aqueous phase, washed with 3 M sodium acetate and
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