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14 resultados
Silencing of the tapetum-specific zinc finger gene TAZ1 causes premature degeneration of tapetum and pollen abortion in petunia.
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TAZ1 (TAPETUM DEVELOPMENT ZINC FINGER PROTEIN1; renamed from PEThy; ZPT3-2) cDNA was first isolated as an anther-specific cDNA from petunia. Here, we report a functional characterization that includes analysis of spatial and temporal expression profiles and examination of anther phenotypes in
DNA degradation and nuclear degeneration during programmed cell death in petals of Antirrhinum, Argyranthemum, and Petunia.
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Programmed cell death (PCD) was studied in the petals of Antirrhinum majus, Argyranthemum frutescens, and Petunia hybrida, using DNA degradation and changes in nuclear morphology as parameters. The petals exhibit loss of turgor (wilting) as a visible symptom of PCD. DNA degradation, as shown on
RNase X2, a pistil-specific ribonuclease from Petunia inflata, shares sequence similarity with solanaceous S proteins.
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Petunia inflata, a species with gametophytic self-incompatibility, has previously been found to contain a large number of ribonucleases in the pistil. The best characterized of the pistil ribonucleases are the products of the S alleles, the S proteins, which are thought to be involved in
[The development of pollen grains and formation of pollen tubes in higher plants : I. Quantitative measurements of the DNA-content of generative and vegetative nuclei in the pollen grain and pollen tube of Petunia hybrida mutants].
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The DNA-content of generative and vegetative nuclei in mature pollen grains of four Petunia hybrida mutants was determined by cytophotometry. In addition the DNA-content of generative and vegetative nuclei in the pollen tube of two of these four mutants (virescens-2 n and ustulata-2 n) was
Calcium in pollen-pistil interaction in Petunia hybrida Hort. I. Localization of Ca2+ ions in mature pollen grain using pyroantimonate and autoradiographic methods.
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The localization of Ca2+ in the mature pollen grain and the flow of these ions from the somatic tissues of the anther to the pollen grains has been studied using pyroantimonate and autoradiographic methods. In the pollen grain, Ca2+ ions have been localized in the sporoderm and in the cytoplasmic
Isolation by PCR of a cDNA clone from pea petals with similarity to petunia and wheat zinc finger proteins.
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The C2H2 TFIIIA/Krüppel class of zinc finger proteins are an important group of regulatory nucleic acid binding factors and have been extensively studied in humans, Drosophila and yeast. We have employed 3' RACE PCR, using a highly degenerate oligonucleotide primer, for the facile isolation of a
Characterization of two ethylene receptors PhERS1 and PhETR2 from petunia: PhETR2 regulates timing of anther dehiscence.
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Two cDNAs (PhERS1 and PhETR2) encoding ethylene receptor homologues were cloned and characterized from petunia. Genomic Southern blot analysis revealed that small families of PhERS1-related and PhETR2-related genes exist in petunia. PhERS1 and PhETR2 are constitutively expressed in stem, flower bud,
In vitro hybridization by sexual methods and by fusion of somatic protoplasts : Experiments with Nicotiana tabacum x Petunia hybrida, N. tabacum x Hyoscyamus niger, H. niger x P. hybrida, Melandrium album x P. hybrida.
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1. No hybrid plants of Nicotiana tabacum + Petunia hybrida were regenerated from calluses of fusion experiments with mesophyll protoplasts of N. tabacum s, s (2) and v and of P. hybrida mu 1 (2). 2. After in vitro pollination of ovules of N. tabacum with pollen of P. hybrida, filamentous proembryos
Characterization of petunia flower mottle virus (PetFMV), a new potyvirus infecting Petunia x hybrida.
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With the introduction of cutting-grown Petunia x hybrida plants on the European market, a new potyvirus which showed no serological reaction with antisera against any other potyviruses infecting petunias was discovered. Infected leaves contained flexuous rod-shaped virus particles of 750-800 nm in
Cloning and characterization of a cDNA encoding a Rab1-like small GTP-binding protein from Petunia hybrida.
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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to
Downregulation of ovule-specific MADS box genes from petunia results in maternally controlled defects in seed development.
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A maternally determined seed defect has been obtained by downregulation of the petunia MADS box genes Floral Binding Protein 7 (FBP7) and FBP11. These genes have been previously shown to play central roles in the determination of ovule identity. Aberrant development of the seed coat and consequent
ABA and IAA control microsporogenesis in Petunia hybrida L.
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The formation of fertile male gametophyte is known to require timely degeneration of polyfunctional tapetum tissue. The last process caused by the programmed cell death (PCD) is a part of the anther program maturation which leads to sequential anther tissue destruction coordinated with pollen
Calcium in pollen-pistil interaction in Petunia hybrida Hort. III. Localization of Ca2+ ions and Ca(2+)-ATPase in pollinated pistil.
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Studies were carried out of CA2+ and CA(2+)-ATPase localization in pollinated (6 and 48 h after pollination) pistils of Petunia hybrida. The results were confronted with CA2+ localization in mature pollen grain and in unpollinated pistil. It has been found that after pollination the number of CA2+
Cloning and expression of flavonol synthase from Petunia hybrida.
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Flavonols are important co-pigments in flower colour and are also essential for pollen tube growth. In petunia, flavonol synthesis is controlled by the Fl locus. Flavonol synthase (FLS) belongs to the 2-oxoglutarate-dependent dioxygenase family. Dioxygenase gene fragments were amplified by PCR on
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