phenylhydrazine/hemorragia

Sustained erythropoiesis and concurrent bone marrow hyperplasia are proposed to be responsible for low bone mass density (BMD) in chronic hemolytic pathologies. As impaired erythropoiesis is also frequent in these conditions, we hypothesized that free heme may alter marrow and bone physiology in

The effect of phenylhydrazine on protein breakdown in rabbit reticulocytes.

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In contrast to reticulocytes produced by bleeding reticulocytes produced by injections of phenylhydrazine exhibit a sizeable Antimycin A- and a large salicylhydroxamic acid (SHAM)-resistant proteolysis. In their cytosol there exist both ATP-independent and ATP-dependent protease systems. The

Impaired adenylate cyclase activity of phenylhydrazine-induced reticulocytes.

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A method which involves Percoll gradient centrifugation, is described for separating rabbit reticulocytes from other blood cells, including erythrocytes. This permits a quantitative comparison of the adenylate cyclase activity of reticulocyte membranes which had been induced either by bleeding (30%

Hemostatic alterations associated with phenylhydrazine-induced anemia in the rat.

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Phenylhydrazine (PHZ) is a hemolytic agent which has been used in the treatment of polycythemia vera. Recent studies performed in our laboratory have indicated that the PHZ-induced anemia is immuno-hemolytic in etiology, and a prolonged bleeding time was present in some of the rats chronically

Effects of phenylhydrazine or phlebotomy on peripheral blood, bone marrow and erythropoietin in Wistar rats.

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This study was conducted to characterize better the response of rats to blood loss and hemolysis and to incorporate automated methods into the routine evaluations of those responses. Serial phlebotomies of 1.5-2.0 ml of blood per day for 5 days, or intraperitoneal injection of 50 mg kg(-1)

Effect of cell age and phenylhydrazine on the cation transport properties of rabbit erythrocytes.

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We studied the effect of cell age on the cation transport systems of rabbit erythrocytes by increasing the proportion of circulating young erythrocytes with either repeated bleeding or with phenylhydrazine (PHZ) treatment. We found that when the reticulocyte content of rabbit blood is increased by

THE EFFECTS OF BLOOD LOSS AND BLOOD DESTRUCTION UPON THE ERYTHROID CELLS IN THE BONE MARROW OF RABBITS.

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The relative proportion of the various sorts of erythroid cells of the bone marrow has been determined after acute and chronic hemorrhage and after damage to the marrow with acetyl phenylhydrazine. The normal erythroid pattern shows megaloblasts and erythroblasts in the lowest percentage, then

Abnormal membrane surface properties during maturation of rat reticulocytes elicited by bleeding as measured by partition in two-polymer aqueous phases.

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Partition of cells in two-polymer aqueous phases is an extremely sensitive method for the separation of cells and for tracing subtle changes in the cells' membrane surface properties (primarily surface charge) as a function of in vivo processes (e.g. differentiation, maturation, ageing). Combining

Haemorrhagic and haemolytic anaemias in the rabbit: a clinically relevant laboratory project in physiology.

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Study of animal models of human anaemias has been part of a laboratory curriculum in physiology for several years. Bleeding or phenylhydrazine injection of rabbits produced anaemias simulating important clinical disorders. Data obtained by the students are given, showing the course over a 14-day

[Role of the kidneys in the mechanism of erythrodieresis in hemolytic (phenylhydrazine) and acute posthemorrhagic anemia].

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The degree of hemolysis was studied comparatively in intact and nephrectomized rats after the phenylhydrazine injection. In the nephrectomized animals hemolytic (phenylhydrazine) anemia was expressed by a lesser reduction of the total erythrocyte count, of the percentage of Cr51-labeled

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