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seminoma/carbohydrate
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Cystic seminoma with elevated value of carbohydrate antigen 19-9 in tumor fluid mimicking cystic teratoma.
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The incidence of cystic lesions of anterior mediastinum is low, which is responsible for approximately 10% of anterior mediastinal tumors. We presented a special case of primary anterior mediastinal tumor with both prominent cystic change and extreme high carbohydrate antigen 19-9 level of cystic
Histochemically demonstrable changes in cell surface carbohydrates of human germ cell tumors.
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Cell surface carbohydrate chains of human germ cell tumors were investigated histochemically using peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin I (UEA-1), and anti-I-(Ma) antibody. Of 16 cases of embryonal carcinoma in adult testis, peanut agglutinin, a
[Ultrastructural cytology of testicular seminomas].
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The aim of this electron microscopic study was to analyze and compare aspects of various histologically distinct seminoma variants including the classical form (11 cases), anaplastic seminoma (1 case) and seminoma with syncytiotrophoblastic giant cells. Sub-microscopic features of these seminoma
Standardization strategy for quantitative PCR in human seminoma and normal testis.
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Housekeeping genes are commonly used as endogenous references in quantitative RT-PCR. Ideally these genes are constitutionally expressed by all cell types and do not vary under experimental conditions. Tissues of 9 normal testes and 22 classical pure seminoma were obtained for RNA-extraction.
Spermatocytic seminoma as compared to classical seminoma: an immunohistochemical and DNA flow cytometric study.
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Based on immunohistochemistry (IHC) and DNA ploidy, different paths of carcinogenesis have been suggested for spermatocytic seminoma (SS) and classical seminoma (CS). The present study extends current knowledge on the above parameters.
METHODS
Seventeen SSs and twenty-two CSs were assessed by IHC
Enzymatic heterogeneity of seminomas.
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Heterogeneity of placental-like alkaline phosphatase (PLAP-like enzyme) in seminoma was studied. PLAP-like enzyme from seminoma tissues was separated into three areas with different proportions between tumors, while PLAP and PLAP-like enzymes in normal testes were separated into two areas on the
Immunohistochemical analysis of LNT, NeuAc2----3LNT, and Lex carbohydrate antigens in human tumors and normal tissues.
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Monoclonal antibodies (MAbs) K21 and K4 define two carbohydrate determinants, lacto-N-tetraose (LNT) and sialylated LNT (NeuAc2----3LNT). respectively, which are expressed on the surface of cultured human teratocarcinoma cells, but not on a wide range of other epithelial and nonepithelial cells in
Lectin binding sites in human seminiferous epithelium, in CIS cells and in seminomas.
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The distribution of glycoconjugates in germ cells during spermatogenic differentiation, in carcinoma-in-situ (CIS) cells and in seminoma were studied by lectin histochemistry. The results show that human germ cells are rich in carbohydrate-containing compounds with specific alterations in the
Hydrophobicity and lectin affinity of alkaline phosphatase isozymes in seminoma and normal testis.
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Isozymes of alkaline phosphatases (ALP) in seminoma and normal testis were separated by use of high-performance liquid chromatography and a TSK-gel phenyl-5PW column. The tissue-nonspecific (liver) ALP (LAP) was the dominating isozyme, consisting of more than 90% ALP activity. The placental ALP
Electrophoretic heterogeneity of alkaline phosphatase isozymes in seminoma and normal testis.
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Electrophoretic patterns of seminoma- and normal-testis-derived alkaline phosphatase isozymes, the placental alkaline phosphatase (PLAP)-like enzyme and the tissue-nonspecific (liver) alkaline phosphatase (LAP), were studied on starch gel and isoelectric focusing (IEF). Different migration patterns
Patterns of seminoma tissue markers and deletions.
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Seminomas and control tissues were analyzed for several tumor markers. Very high levels of placental alkaline phosphatase (PLAP)-like enzyme levels were found in all 18 seminomas studied. The majority of the seminomas were of phenotype I, thus differing from palcental PLAP. The mean amount of enzyme
Characterization of seminoma-derived placental-like alkaline phosphatase.
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Characteristics of placental-like alkaline phosphatase (PLAP-like enzyme) in seminoma was studied. By use of lectin affinity chromatography, PLAP-like enzyme in seminoma revealed extra sugar chains compared to placental alkaline phosphatase (PLAP), indicating heterogeneity of the carbohydrate
Identification of key genes and pathways in seminoma by bioinformatics analysis.
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Background: Seminoma accounts for the most part of cases of testicular germ cell tumor, which is the most common malignancy among males between ages 15 and 44 years. Understanding the molecular mechanism of tumorigenesis is important for better clinical diagnosis and treatment.
Tumor-associated carbohydrate antigens defining tumor malignancy: basis for development of anti-cancer vaccines.
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Tumors expressing a high level of certain types of tumor-associated carbohydrate antigens (TACAs) exhibit greater metastasis and progression than those expressing low level of TACAs, as reflected in decreased patient survival rate. Well-documented examples of such TACAs are: (i) H/Le(y)/Le(a) in
A new small cell lung cancer (SCLC)-specific marker discovered through antigenic subtraction of neuroblastoma cells.
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Small cell lung cancer (SCLC) is an aggressive form of lung cancer associated with cigarette smoking and presently accounts for approximately 20% of all lung cancer cases. SCLC cells derive from a neuroendocrine origin and therefore their antigenic profile coincides, to a great extent, with that of
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