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12 resultados
The effect of taxol and ethyl-N-phenylcarbamate (EPC) on growth and gravitropism in Zea mays L.
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The effect of taxol and ethyl-N-phenylcarbamate (EPC) on the growth and gravitropism of maize roots and coleoptiles was studied. Taxol is known to promote the assembly of microtubules (MTs) and stabilizes MTs by preventing depolymerization. EPC, on the contrary, is an anti-microtubule drug that
Cortical actin filaments potentially interact with cortical microtubules in regulating polarity of cell expansion in primary roots of maize (Zea mays L.).
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Evidence is accumulating implicating cortical microtubules in the directional control of cell expansion. However, the role of actin filaments in this process is still uncertain. To determine the involvement of actin in cell elongation, the organization of actin filaments in primary roots of maize
Disruption of actin filaments in Zea mays by bisphenol A depends on their crosstalk with microtubules.
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Bisphenol A (BPA) is a widespread environmental pollutant, reportedly harmful to living organisms. In plant cells, BPA was shown to disrupt microtubule (MT) arrays and perturb mitosis, but its effects on filamentous actin (F-actin) have not been explored. Here we studied the effects of BPA on actin
Rapid and Reversible High-Affinity Binding of the Dinitroaniline Herbicide Oryzalin to Tubulin from Zea mays L.
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Oryzalin, a dinitroaniline herbicide, was previously reported to bind to plant tubulin with a moderate strengthe interaction (dissociation constant [Kd] = 8.4 [mu]M) that appeared inconsistent with the nanomolar concentrations of drug that cause the loss of microtubules, inhibit mitosis, and produce
Characterization of the reversible taxol-induced polymerization of plant tubulin into microtubules.
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Taxol has been reported to induce the polymerization of plant tubulin into microtubules, albeit weakly when compared to that of mammalian tubulin [Morejohn, L.C., & Fosket, D.E. (1984) J. Cell Biol. 99, 141-147], suggesting that taxol, a product of plant secondary metabolism, may interact poorly
Abundance of actin filaments in the preprophase band and mitotic spindle of brick1 Zea mays mutant.
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The preprophase band and mitotic spindle of dividing protodermal cells of wild-type Zea mays leaves include few actin filaments. Surprisingly, abundant actin filaments were observed in the above arrays, in dividing protodermal cells in the leaves of the brick1 mutant. The same abundance was observed
Gravitropism of the primary root of maize: a complex pattern of differential cellular growth in the cortex independent of the microtubular cytoskeleton.
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The spatio-temporal sequence of cellular growth within the post-mitotic inner and outer cortical tissue of the apex of the primary root of maize (Zea mays L.) was investigated during its orthogravitropic response. In the early phase (0-30 min) of the graviresponse there was a strong inhibition of
Polyethylene glycol modified phospholipids stabilize emulsions prepared from triacylglycerol.
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A stable lipid-based carrier system containing a triacylglycerol core has been developed. This has been achieved by homogenization of corn oil (primarily triacylglycerol) in the presence of phosphatidylcholine (PC), preformed 100 nm liposomes prepared from PC/cholesterol (55: 45; mol:mol) and
Formation of an endoplasmic reticulum ring associated with acetylated microtubules in the angiosperm preprophase band.
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We investigated the organization of the cortical endoplasmic reticulum (ER) in prophase cells of the angiosperms Zea mays, Triticum turgidum, and Vigna sinensis. In both symmetrically and asymmetrically dividing protodermal leaf cells, cortical ER was enriched in the preprophase band and colocalized
The microtubule cytoskeleton does not integrate auxin transport and gravitropism in maize roots.
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The Cholodny-Went hypothesis of gravitropism suggests that the graviresponse is controlled by the distribution of auxin. However, the mechanism of auxin transport during the graviresponse of roots is still unresolved. To determine whether the microtubule (MT) cytoskeleton is participating in auxin
Biochemical characterization and molecular dynamic simulation of β-sitosterol as a tubulin-binding anticancer agent.
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Βeta-sitosterol (β-SITO), a phytosterol present in pomegranate, peanut, corn oil, almond, and avocado, has been recognized to offer health benefits and potential clinical uses. β-SITO is orally bioavailable and, as a constituent of edible natural products, is considered to have no undesired side
Diet modulation is an effective complementary agent in preventing and treating breast cancer lung metastasis.
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A significant percentage of breast cancer victims will suffer from metastases indicating that new approaches to preventing breast cancer metastasis are thus needed. Dietary stearate (ST) and chemotherapy have been shown to reduce breast cancer metastasis. We tested the complementary use of dietary
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