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8 resultados
Thiamine requirement in relation to cytokinin in "normal" and "mutant" strains of tobacco callus.
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High concentrations of kinetin (400-2,000 μg/l) permit continuous growth of tobacco callus cultures (Nicotiana tabacum, var. Wisconsin No. 38) in the absence of exogenous thiamine. On the optimum concentration (1,000 μg/l) the tissue has been maintained through 21 bimonthly passages without change
[Activation of thiamine biosynthesis in callus cultures of Nicotiana Tabacum in the light].
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In darkness growth of callus tissues of Nicotiana tabacum var. "Samsun" depends on an exogenous supply of thiamine; without addition of thiamine growth decreases and after 4 to 5 passages of three weeks on a thiaminefree medium the tissues die. In contrast to this result, the same tissue has been
A Nuclear Mutation in Nicotiana sylvestris Causing a Thiamine-Reversible Defect in Synthesis of Chloroplast Pigments.
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We report the recovery of a nuclear recessive mutation in Nicotiana sylvestris (Spegazzini and Comes) producing a conditional disruption in the pathway for synthesis of chlorophyll a and b and carotenoids which is fully reversible by exogenous thiamine (0.3 micromolar). In the absence of
Overexpression of plastid transketolase in tobacco results in a thiamine auxotrophic phenotype.
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To investigate the effect of increased plastid transketolase on photosynthetic capacity and growth, tobacco (Nicotiana tabacum) plants with increased levels of transketolase protein were produced. This was achieved using a cassette composed of a full-length Arabidopsis thaliana transketolase cDNA
Comparison of de-novo flower-bud formation in a photoperiodic and a day-neutral tobacco.
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Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l
Photosynthetic characterization of photoautotrophic cells cultured in a minimal medium.
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Photosynthetic properties of photoautotrophic suspensions cultured in a minimal growth medium have been evaluated to determine whether changes have occurred in ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity, phosphoenol-pyruvate (PEP) carboxylase activity, chlorophyll content, or
Properties of mutant acetolactate synthases resistant to triazolopyrimidine sulfonanilide.
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Triazolopyrimidine sulfanilides are a class of highly active herbicides whose primary target is acetolactate synthase. Spontaneous mutants of tobacco (Nicotiana tabacum) (KS-43) and cotton (Gossypium hirsutum) (PS-3 and DO-2) resistant to triazolopyrimidine sulfonanilide were selected in tissue
Verticillium dahliae VdTHI20, Involved in Pyrimidine Biosynthesis, Is Required for DNA Repair Functions and Pathogenicity.
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Verticillium dahliae (V. dahliae) infects roots and colonizes the vascular vessels of host plants, significantly reducing the economic yield of cotton and other crops. In this study, the protein VdTHI20, which is involved in the thiamine biosynthesis pathway, was characterized by
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