BioMed Research International 2020
Changes of Differential Urinary Metabolites after High-Intensive Training in Teenage Football Players.
Numai utilizatorii înregistrați pot traduce articole
Log In / Înregistrare
Linkul este salvat în clipboard
Cuvinte cheie
Abstract
Methods
12 teenage football players were subjected to three groups of combined training by using a cycle ergometer, with the subjective Rating of Perceived Exertion (RPE) as a fatigue criterion. The following indicators were measured in each group after training: maximum oxygen uptake (VO2max), anaerobic power, and average anaerobic power. Urine samples were collected before and after the training. Gas chromatography-mass spectrometry (GC-MS) was performed for the metabonomics analysis of the samples. The metabolism data was analyzed by using principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA), through the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to confirm the potential differences between metabolites, and the MetPA database was used to analyze the related metabolic pathways.Results
There was no significant difference between the maximal oxygen uptakes among the three groups. Compared with group 1, the maximum and average anaerobic power in group 3 significantly decreased (p < 0.05) at the end of training. GC-MS detected 635 metabolites in the urine samples. Through PCA, OPLS-DA analysis, and KEGG matching, 25 different metabolites (3↑22↓) that met the conditions were finally selected. These different metabolites belonged to 5 metabolic pathways: glycine-serine-threonine metabolism, citrate cycle, tyrosine metabolism, nitrogen metabolism, and glycerophospholipid metabolism.