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Relationship between Ureide N and N(2) Fixation, Aboveground N Accumulation, Acetylene Reduction, and Nodule Mass in Greenhouse and Field Studies with Glycine max L. (Merr).

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The relationship between ureide N and N(2) fixation was evaluated in greenhouse-grown soybean (Glycine max L. Merr.) and lima bean (Phaseolus lunatus L.) and in field studies with soybean. In the greenhouse, plant N accumulation from N(2) fixation in soybean and lima bean correlated with ureide N.

Effect of protein additives on acetylene reduction (nitrogen fixation) by Rhizobium in the presence and absence of soybean cells.

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The effect of protein additives on acetylene reduction (N(2) fixation) by Rhizobium associated with soybean cells (Glycine max [L.] Merr.) in vitro was studied. Acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of N/ml supplied as aqueous extracts of hexane-extracted

Adaptation of Nodulated Soybean (Glycine max L. Merr.) to Growth in Rhizospheres Containing Nonambient pO(2).

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Nodulated soybean (Glycine max L. Merr. cv White Eye inoculated with Bradyrhizobium japonicum strain CB 1809) plants were cultured in the absence of combined N from 8 to 28 days with their root systems maintained continuously in 1, 2.5, 5, 10, 20, 40, 60, or 80% O(2) (volume/volume) in N(2). Plant

Proline fed to intact soybean plants influences acetylene reducing activity and content and metabolism of proline in bacteroids.

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Supplying l-proline to the root system of intact soybean (Glycine max [L.] Merr.) plants stimulated acetylene reducing activity to the same extent as did supplying succinate. Feeding l-proline also caused an increase in bacteroid proline dehydrogenase activity that was highly correlated with the

Simultaneous Measurement of NH(4) Absorption and N(2) Fixation by Glycine max L. : RESPONSE TO TEMPERATURE, pH, AND EXTERNAL NITROGEN CONCENTRATION.

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Ammonium absorption rates by intact nodulated and unnodulated soybean plants (Glycine max [L.] Merr., Amsoy 71) were determined from the liquid phase of a mist assay chamber. From the gas phase, simultaneous measurements of acetylene reduction rates were made from nodulated plants. Ammonium

Synergetic Cultures of Glycine max Root Cells and Rhizobia Separated by Membrane Filters.

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When suspension cultures of actively growing soybean (Glycine max L.) root cells were separated by two or three membrane filters from suspension cultures of the bacteria, a synergetic (cooperative) activation of nitrogenase was observed in the Rhizobium japonicum used in the bacterial side. Either

Acetylene reduction (nitrogen fixation) and metabolic activities of soybean having various leaf and nodule water potentials.

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An apparatus was designed that permitted acetylene reduction (N(2) fixation) by root nodules to be measured in situ simultaneously with net photosynthesis, dark respiration, and transpiration of the shoot in soybean plants (Glycine max [L.] Merr. var. Beeson). Tests showed that acetylene reduction

Limitation of acetylene reduction (nitrogen fixation) by photosynthesis in soybean having low water potentials.

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The role of photosynthesis and transpiration in the desiccation-induced inhibition of acetylene reduction (nitrogen fixation) was investigated in soybean (Glycine max [L.] Merr. var. Beeson) using an apparatus that permitted simultaneous measurements of acetylene reduction, net photosynthesis, and

Effect of acetylene on root respiration and acetylene reducing activity in nodulated soya bean.

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Acetylene decreased root and nodule respiration, as measured by CO(2) evolution of nodulated or non-nodulated Glycine max. An inhibition of 25 to 35% in 15 to 30 minutes occurred when 13% C(2)H(2) was introduced in the gas flux which aerated the root nutrient solution. When the light intensity was

Continuous, automated acetylene reduction assays using intact plants.

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An automated method was developed for continuous, in situ determination of acetylene reduction (N(2) fixation) by intact soybean plants (Glycine max [L.]). The culture vessel containing the roots of intact plants grown in sand culture is sealed at the surface and an air-acetylene mixture

Effect of light, dark, and temperature on root nodule activity (acetylene reduction) of soybeans.

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The objectives of this study were (a): to define the effects of light, dark, and temperature on nodule activity (acetylene reduction), and (b) to establish the contributions of reserve carbohydrate and recent photosynthate to the support of nodule function. An in situ assay of nodule activity was

Comparing time course profiles of immediate acetylene reduction by grasses and legumes.

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The time course profiles of C(2)H(2) reduction by intact Scirpus olneyi (bulrush), Oryza sativa (rice) and Spartina alterniflora (cordgrass) with roots in atmospheres of N(2) and 30-day-old Glycine max (soybean) in air were all immediately linear. This is the first report of immediately linear rates

Purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (Glycine max Merr) nodules.

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A non-haem iron protein was isolated from an extract of soya-bean nodule bacteroids by a procedure including protamine sulphate and heat precipitation followed by chromatography on DEAE-cellulose. The purified protein contains non-haem iron and acid-labile sulphur and exhibits a spectrum with a

Acetylene reduction by soil cores of maize and sorghum in Brazil.

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Nitrogenase activity was measured by the C(2)H(2) reduction method in large soil cores (29 cm in diameter by 20 cm in depth) of maize (Zea mays) and sorghum (Sorghum vulgare). The activity was compared to that obtained by a method in which the roots were removed from the soil and assayed for

Simultaneous measurement of nitrogen fixation estimated by acetylene-ethylene assay and nitrate absorption by soybeans.

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An apparatus was designed for simultaneous measurement of rates of N(2) fixation estimated by C(2)H(2)-C(2)H(4) assay (N(2)[C(2)H(2)] fixation) and NO(3) (-) absorption by roots of intact, nodulated soybeans (Glycine max [L.] Merr.). The principal design features include: (a) a gas-tight mist

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