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Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes.

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Steroidal glycoalkaloids (SGAs) such as α-solanine found in solanaceous food plants--as, for example, potato--are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA

Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight.

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Genetic transformation with resistance (R) genes is expected to enhance resistance durability against pathogens, especially for potato, a vegetatively propagated crop with tetrasomic inheritance and a long-term breeding program. In this study, 128 potato transformants were analysed for the presence

Development of allele-specific PCR and RT-PCR assays for clustered resistance genes using a potato late blight resistance transgene as a model.

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Members of the NBS-LRR gene family impart resistance to a wide variety of pathogens and are often found clustered within a plant genome. This clustering of homologous sequences can complicate PCR-based characterizations, especially the study of transgenes. We have developed allele-specific PCR and

A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans.

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Phytophthora infestans is the cause of late blight, a devastating and re-emerging disease of potato. Significant advances have been made in understanding the biology of P. infestans, and in the development of molecular tools to study this oomycete. Nevertheless, little is known about the molecular

Streptomyces species isolated from potato scabs in France: numerical analysis of "Biotype-100" carbon source assimilation data.

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Thirty one strains of Streptomyces isolated from common and netted scabs in France were characterized using "Biotype-100" strips and 14 conventional tests. Numerical analysis showed that S. scabiei was the single causal agent of common scab in France. However the strains clustered among S. scabiei

Phylogenetic analyses of an isolate obtained from potato in 1985 revealed potato virus X was introduced to China via multiple events.

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Potato virus X (PVX) is one of the most common plant viruses that cause great economic losses to solanaceous plants. We have previously reported the complete genomic sequence of the 2006 Chinese potato isolate FX21 and demonstrated that PVX isolates cluster into two groups: Eurasia and America.

Identification and expression analysis of aquaporins in the potato psyllid, Bactericera cockerelli.

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Aquaporin (AQPs) proteins transport water and uncharged low molecular-weight solutes across biological membranes. Six to 8 AQP genes have been identified in many insect species, but presently only three aquaporins have been characterized in phloem feeding insects. The objective of this study was to

Phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants.

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Rhizobacteria obtained during a risk assessment study from parental and transgenic T4 lysozyme-expressing potato plants were investigated to determine whether or not the strains could be grouped based on the source of isolation, transgenic or non-transgenic plants, respectively. A total of 68

Genetic Variability in the Potato Pathogen Colletotrichum coccodes as Determined by Amplified Fragment Length Polymorphism and Vegetative Compatibility Group Analyses.

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ABSTRACT Amplified fragment length polymorphism (AFLP) using three primer sets was used to characterize 211 Colletotrichum coccodes isolates from North America, 112 of which were assigned to six vegetative compatibility groups (VCGs) using nitrate nonutilizing (nit) mutants. These isolates clustered

Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea.

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The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their

A member of the Hsp60 gene family from the peach potato aphid, Myzus persicae (Sulzer.).

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A novel cDNA clone encoding a mitochondrial Hsp60 was isolated from a Myzus persicae cDNA library. The nucleotide sequence consisted of 2348 bp and contained an open reading frame (ORF) of 1722 bases. The putative protein encoded by this ORF consisted of 574 amino acids and was designated MPHSP60.

New features on the genomic organization of a novel dicistrovirus identified from the sweet potato whitefly Bemisia tabaci

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The whitefly Bemisia tabaci is an agricultural pest causing large economic losses worldwide. We analysed the genomic sequence of a new viral member of the family Dicistroviridae identified by high-throughput sequencing of total RNA extracted from whiteflies. The virus, tentatively named

Molecular characterization of two recombinant potato virus Y isolates from China.

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Isolates of potato virus Y (PVY) have been divided into several strains. We determined the genomic sequences of PVY isolates AQ4 and FZ10 from tobacco in China. AQ4 and FZ10 had genome of 9700 and 9698 nucleotides, respectively. In phylogenetic analysis of complete genome sequences, AQ4 was

Cytogenetic and molecular characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae.

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Electrophoresis following digestion of Myzus persicae genomic DNA with HindIII showed the presence of a prominent band of approximately 200 bp whereas a faint electrophoretic band corresponding to DNA fragments of about 3000 bp was observed after digestion with ApaI. In situ digestion with

Genome-wide analysis of the MATE gene family in potato.

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The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of antibiotics out of the cell using an electrochemical gradient of H+ or Na+ across the membrane. The main function of MATE gene family

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