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Survival and concanavalin-A-induced capping in CHO fibroblasts after exposure to hyperthermia, ethanol, and X irradiation.

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Effect of colchicine on immunoregulatory abnormalities in familial Mediterranean fever.

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The effect of colchicine on immunoregulatory T lymphocytes in children with familial Mediterranean fever (FMF) was studied. Concanavalin A (Con A)-induced suppressor cell function was significantly (P less than 0.0001) decreased in five untreated FMF patients (15 +/- 3%, mean +/- s.e.) as compared

Effects of in vitro hyperthermia on human natural killer cells.

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The present study demonstrates that human natural killer (NK) cells isolated from peripheral blood of normal individuals are highly sensitive to hyperthermia. The effect was time and dose dependent, and treatment of peripheral blood lymphocytes at 42 degrees for 1 hr almost completely abolished NK

Stromal macrophage-histiocytes in Hodgkin's disease. Their relation to fever.

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Morphologic variations of Concanavalin A-binding histiocytes were studied in biopsy specimens of 140 untreated patients with Hodgkin's disease (72 asymptomatic, and 68 with constitutional symptoms). Fever was the most common symptom, present in 57 of the 68 patients. Three morphologic types of

Suppressor cell function in a family with familial Mediterranean fever.

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Defective suppressor cell function has been demonstrated in several diseases but has not been tested in familial Mediterranean fever (FMF). We tested the ability of concanavalin A-activated suppressor cells from one family with FMF to inhibit the proliferation of phytohaemagglutinin-stimulated

Correction of a suppressor cell deficiency in familial Mediterranean fever by colchicine.

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We have previously reported a suppressor cell deficiency in four patients with familial Mediterranean fever (FMF). Since colchicine prevents FMF attacks, we tested the effect of colchicine (1 mg twice daily) on the suppressor cell function in three of these FMF patients. Proliferation of

Lymphocyte apoptosis during classical swine fever: implication of activation-induced cell death.

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Infection of pigs with classical swine fever virus (CSFV), a member of the Flaviviridae family, causes a severe leukopenia, particularly notable with the lymphocytes. The goal of this study was to analyze mechanisms behind this CSFV-induced lymphopenia. To this end, the kinetics of leukocyte

In vivo and in vitro effects of moderately virulent African swine fever virus on mitogenesis of pig lymphocytes.

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Six pigs were infected oro-nasally with a moderately virulent African swine fever (ASF) virus from the Dominican Republic (DR II). The effect of virus infection on the pig's immune system was tested by measuring peripheral leucocyte numbers and the ability of mononuclear leucocytes (MNL) to respond

Precipitating antibodies to non-treated dengue type 2 viral antigens in sera of Thai hemorrhagic fever patients by crossed immunoelectrophoresis.

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Crossed immunoelectrophoresis of dengue type 2 virus revealed at least two precipitating antigens which shared some antigenic determinants. Glycoprotein components of both antigens were detected by binding to concanavalin A. Sera from dengue hemorrhagic fever patients showed precipitating antibodies

Seasonal variation in suppressor T cell subsets and non-specific suppressor cell function in hay fever sufferers.

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The helper/suppressor T cell ratio, as defined by monoclonal antibodies, was significantly higher in hay fever sufferers compared with controls (P less than 0.05), but only during or shortly after the pollen season. This was due to a reduction in the suppressor subset, which returned to control

[Cellular and humoral immunity in general exogenous hyperthermia].

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The proliferative activity of splenic cells (SC) to phytohemagglutinin (PHA), concanavalin A (Con A), lipopolysaccharide (LPS), pokeweed mitogen (PWM) and alloantigens, as well as the immune response to sheep red cells (SRS) were investigated in mice undergoing hyperthermia. There was an increase in

Effect of antibiotics on clinical, pathologic and immunologic responses in murine Potomac horse fever: protective effects of doxycycline.

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Effects of three antibiotics on clinical, pathologic and immunologic responses in murine Potomac horse fever caused by Ehrlichia risticii infection were examined. When antibiotics were given after the development of clinical signs, antibiotics ranked in the order of reducing clinical signs and in

Heat shock impairs DNA synthesis and down-regulates gene expression for leptin and Ob-Rb receptor in concanavalin A-stimulated bovine peripheral blood mononuclear cells.

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This study verified whether leptin or its long isoform receptor (Ob-Rb) genes are expressed in proliferating lymphocytes from bovine species, and whether their expression changes with increased temperatures. Peripheral blood mononuclear cells (PBMC) from five Holstein cows were incubated in the

Interaction of classical swine fever virus with membrane-associated heparan sulfate: role for virus replication in vivo and virulence.

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Passage of native classical swine fever virus (CSFV) in cultured swine kidney cells (SK6 cells) selects virus variants that attach to the surface of cells by interaction with membrane-associated heparan sulfate (HS). A Ser-to-Arg change in the C terminus of envelope glycoprotein E(rns) (amino acid

Synthesis of African swine fever (ASF) virus-specific antibodies in vitro in a porcine leucocyte system.

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We have developed a system of porcine peripheral blood mononuclear cells for the synthesis of antibodies in vitro, induced by partially purified African swine fever virus particles inactivated with formaldehyde. The antibodies synthesized were detected by a radioimmunoassay with a sensitivity of 3

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