hordeum distichon/prolină

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Oxidation of proline by plant mitochondria.

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Mitochondria isolated from etiolated shoots of corn (Zea mays), wheat (Triticum aestivum), barley (Hordeum vulgare), soybean (Glycine max L. Merr.), and mung bean (Phaseolus aureus) exhibited a proline-dependent O(2) uptake subject to respiratory control. ADP/O ratios with proline as substrate were

Uptake of proline by the scutellum of germinating barley grain.

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Scutella separated from germinating grains of barley (Hordeum vulgare L. cv Himalaya) took up 1 millimolar l-[(14)C]proline at an initial rate of about 6.5 micromoles gram(-1) fresh weight hour(-1) (pH 5, 30 degrees C). The uptake had a pH optimum at 5. The bulk of the uptake (93%) was via

Mobilization of proline in the starchy endosperm of germinating barley grain.

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In germinating grains of barley, Hordeum vulgare L. cv. Himalaya, free proline accumulated in the starchy endosperm during the period of rapid mobilization of reserve proteins. When starchy endosperms were separated from germinating grains and homogenized in a dilute buffer of pH 5 (the pH of the

Relationship between Stress-Induced ABA and Proline Accumulations and ABA-Induced Proline Accumulation in Excised Barley Leaves.

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When excised second leaves from 2-week-old barley (Hordeum vulgare var Larker) plants were incubated in a wilted condition, abscisic acid (ABA) levels increased to 0.6 nanomole per gram fresh weight at 4 hours then declined to about 0.3 nanomole per gram fresh weight and remained at that level until

Changes in foliar proline concentration of osmotically stressed barley.

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The amino acid proline is accumulated in plant tissues in response to a variety of stresses. The existence of two routes for its biosynthesis is well documented. However, little is known about the contribution of each pathway to the accumulation of free proline under stress conditions. In the

Metabolism of [5-h]proline by barley leaves and its use in measuring the effects of water stress on proline oxidation.

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The objective of these experiments was to determine the fate of tritium from the 5 position of proline and to assess the validity of its loss to H(2)O as a measure of proline oxidation. When [5-(3)H]proline was fed to barley (Hordeum vulgare) leaves, tritium was recovered in H(2)O and metabolites

Light stimulation of proline synthesis in water-stressed barley leaves.

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The effect of light on [(14)C]glutamate conversion to free proline during water stress was studied in attached barley (Hordeum vulgare L.) leaves which had been trimmed to 10 cm in length. Plants at the three-leaf stage were stressed by flooding the rooting medium with polyethylene glycol 6000

Proline Accumulation in Water-stressed Barley Leaves in Relation to Translocation and the Nitrogen Budget.

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Mobilization of N from leaves of barley (Hordeum vulgare L.) during water stress, and the role of proline as a mobilized species, were examined in plants at the three-leaf stage. The plants responded to water stress by withdrawing about 25% of the total reduced N from the leaf blades via phloem

Role of carbohydrates in proline accumulation in wilted barley leaves.

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The effect of wilting on proline synthesis, proline oxidation, and protein synthesis-all of which contribute to proline accumulation-was determined in nonstarved barley (Hordeum vulgare L.) leaves. Nonstarved leaves were from plants previously in the light for 24 hours and starved leaves were from

Proline accumulation in a barley mutant resistant to trans-4-hydroxy-L-proline.

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Five proline analogues were tested for inhibition of the growth of mature barley (Hordeum vulgare L.) embryos in sterile culture. Inhibition by all analogues was relieved by proline. Inhibition by trans-4-hydroxy-L-proline was relieved by low amounts of proline. Twenty thousand mature embryos were

Effects of the Proline Analog l-Thiazolidine-4-carboxylic Acid on Proline Metabolism.

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The effect of various proline analogs on proline oxidation in mitochondria isolated from etiolated barley (Hordeum vulgare) shoots was investigated. Of the analogs tested, only l-thiazolidine-4-carboxylic acid (T4C) was an effective inhibitor. T4C (1 millimolar) inhibited proline (10 millimolar)

AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgare L.).

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BACKGROUND Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy

Contribution of Arginine to Proline Accumulation in Water-stressed Barley Leaves.

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Barley (Hordeum vulgare cv Prior) leaves converted l-U-(14)C-arginine to labeled proline. Accumulation of radioactivity in proline was greater in wilted leaves, but only after 9 hours of incubation. As the increase in free proline was detectable after only 3 to 6 hours, it is likely that the

Inhibition of proline oxidation by water stress.

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The conversion of proline to glutamic acid and hence to other soluble compounds (proline oxidation) proceeds readily in turgid barley (Hordeum vulgare) leaves and is stimulated by higher concentrations of proline. This suggests that proline oxidation could function as a control mechanism for

Oxidation of proline decreases immunoreactivity and alters structure of barley prolamin.

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Elimination of celiac-toxic prolamin peptides and proteins is essential for Triticeae products to be gluten-free. Instead of enzymatic hydrolysis, in this study we investigated metal-catalyzed oxidation of two model peptides, QQPFP, and PQPQLPY, together with a hordein isolate from barley (Hordeum

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