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A papain-induced disc degeneration model for the assessment of thermo-reversible hydrogel-cells therapeutic approach.

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Nucleus pulposus (NP) regeneration by the application of injectable cell-embedded hydrogels is an appealing approach for tissue engineering. We investigated a thermo-reversible hydrogel (TR-HG), based on a modified polysaccharide with a thermo-reversible polyamide [poly(N-isopropylacrylamide),

Papain-induced in vitro disc degeneration model for the study of injectable nucleus pulposus therapy.

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BACKGROUND Proteolytic enzyme digestion of the intervertebral disc (IVD) offers a method to simulate a condition of disc degeneration for the study of cell-scaffold constructs in the degenerated disc. OBJECTIVE To characterize an in vitro disc degeneration model (DDM) of different severities of

Increased friction of animal joints by experimental degeneration and recovery by addition of hyaluronic acid.

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OBJECTIVE: To investigate the lubricating ability in osteoarthritic (OA) stifle joints. DESIGN: An experimental study in rabbit stifles in vitro. BACKGROUND: The lubricating ability in OA joints, and the effects of the application of hyaluronic acid (HA), a drug for OA, to the osteoarthritic joints

Bovine explant model of degeneration of the intervertebral disc.

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BACKGROUND Many new treatments for degeneration of the intervertebral disc are being developed which can be delivered through a needle. These require testing in model systems before being used in human patients. Unfortunately, because of differences in anatomy, there are no ideal animal models of

Antibacterial activity of papain and bromelain on Alicyclobacillus spp.

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Alicyclobacillus spp. are spore forming bacteria that are often related to the deterioration of acidic products such as beverages and citrus juices. After the process of industrial pasteurization, the spore produced by the bacteria can germinate and the microorganism can grow, causing sensory

[Study of therapeutic effect and mechanism of Sihuang powder treating acute synovitis in experimental rabbit induced by papain injection].

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OBJECTIVE To prove the therapeutic effects of Sihuang powder (composed by four traditional Chinese herbs: root of baikal skullcap, bark of amur corktree, root of sorrel rhubarb, fruit of cape jasmine, which were mixed with wild Chrysanthemum flower solution)in treating acute synovitis in

An ultrastructural study on the ear cartilage of rabbits after the administration of papain. Appearance of cross-striated collagen segments of an atypical FLS-type.

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Crude papain was administered intravenously to young rabbits and the cartilage of the collapsed ear was examined electron-microscopically. Degeneration and recovery of chondrocytes, and decrease in and recovery of the electron-density of elastic fibers, were observed during the collapse and

[A study on papain-induced osteoarthritis in rabbit temporomandibular joint].

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OBJECTIVE The aim of this study was to set up an animal model of osteoarthritis (OA) in the rabbit temporomandibular joint (TMJ). METHODS 0.3 ml of 1.6% papain solution was injected into the upper compartment of each left TMJ of six Japanese big ear white rabbits, and the same volume of saline was

[Effect of SL-1010 (sodium hyaluronate with high molecular weight) on experimental osteoarthritis induced by intra-articularly applied papain in rabbits].

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Sodium hyaluronate (HA) with a molecular weight of approximately 600,000-1,200,000 is reportedly effective against osteoarthritis (OA). However, since HA with higher molecular weight is expected to be more effective against OA, we investigated the effects of HA (SL-1010) newly produced by

Fresh immature articular cartilage allografts. A study on the integration of chondral and osteochondral grafts both in normal and in papain-treated knee joints of rabbits.

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The integration of immature cartilage allografts to knee joints of both normal rabbits and those submitted to arthritis through papain was studied for a period of up to 9 months. Two types of grafts were compared: chondral--constituted only by cartilage, and osteochondral--cartilage plus a thin

Experimental study of pulmonary emphysema in rats exposed to coal dust and papain: effects on the infrastructure and the cell dynamics.

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Experimental lesions of emphysema were produced in SPF rats after laryngotracheal injection of papain and coal dust. The infrastructural changes were investigated a different intervals. The lesions observed are progressive, characterized by atrophy and ruptures of the interalveolar septa, and the

The reaction of cartilage and osteophyte formation after the intraarticular injection of papain.

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A histological and autoradiographical study on the reaction of chondrocytes and the growing processes of osteophyte after the intraarticular injection of papain was carried out. The degenerating processes--fibrillation, death of chondrocytes and erosion--were observed as early as 1 day after the

Multiparametric MR Investigation of Proteoglycan Diffusivity, T2 Relaxation, and Concentration in an Ex Vivo Model of Intervertebral Disc Degeneration.

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Proteoglycan (PG) is a major component of the intervertebral disc extracellular matrix (ECM) that acts to hydrate the disc nucleus. Early detection of PG degradation is valuable for both diagnosis and preclinical research of intervertebral disc degeneration

T1ρ magnetic resonance imaging quantification of early articular cartilage degeneration in a rabbit model.

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BACKGROUND Osteoarthritis (OA) is a serious problem in the recent aging society, and early diagnosis and intervention of articular cartilage degeneration are very important for the onset of OA. Therefore, development of newer MRI techniques is necessary and expected for detection of early articular

[Comparison study on knee osteoarthritis in rabbits induced by different concentrations of papain].

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OBJECTIVE To compare the knee osteoarthritis (OA) models in rabbits by different concentrations of papain and provide data for exploring pathogenesis and treatments of this disease. METHODS Sixty New Zealand white rabbits were randomly divided into four groups of 15 each and given injections into

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