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Na⁺/K⁺ exchange switches the catalytic apparatus of potassium-dependent plant L-asparaginase.

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Plant-type L-asparaginases, which are a subclass of the Ntn-hydrolase family, are divided into potassium-dependent and potassium-independent enzymes with different substrate preferences. While the potassium-independent enzymes have already been well characterized, there are no structural data for

Distribution and Properties of a Potassium-dependent Asparaginase Isolated from Developing Seeds of Pisum sativum and Other Plants.

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Asparaginase (EC 3.5.1.1) was isolated from the developing seed of Pisum sativum. The enzyme is dependent upon the presence of K(+) for activity, although Na(+) and Rb(+) may substitute to a lesser extent. Maximum activity was obtained at K(+) concentrations above 20 millimolar. Potassium ions

Structural basis of potassium activation in plant asparaginases.

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l-asparaginases (EC 3.5.1.1) play an important role in nitrogen mobilization in plants. Here, we investigated the biochemical and biophysical properties of potassium-dependent (PvAspG1) and potassium-independent (PvAspG-T2) l-asparaginases from Phaseolus vulgaris. Our previous studies revealed that

Cell walls as reservoirs of potassium ions for reversible volume changes of pulvinar motor cells during rhythmic leaf movements.

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The laminar pulvinus of primary leaves of Phaseolus coccineus L. was investigated with respect to the total K(+) content, the apoplastic K(+) content, and the water potential of extensor and flexor sections in relation to the leaf positions in a circadian leaf-movement cycle, as well as the

Influence of calcium on sodium and potassium absorption by fresh and aged bean stem slices.

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The influence of Ca on the aging processes of bean stem (Phaseolus vulgaris) slices and on the absorption of K and Na by fresh and aged slices was investigated. In the presence of Ca, fresh tissue showed a preferential Na uptake. The preference for Na over K resulted from a differential depressive

Opposing effects of aluminum on inward-rectifier potassium currents in bean root-tip protoplasts.

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Inward currents in root cap protoplasts of the aluminum-tolerant cultivar, Dade, of Phaseolus vulgaris L. were investigated using the whole-cell patch-clamp technique. The properties of these currents were similar to those seen in inward rectifying K+ channels in other plant tissues. Replacing bath

Light-induced phase shifts of circadian leaf movements of phaseolus: comparison with the effects of potassium and of ethyl alcohol.

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Leaves of Phaseolus coccineus were exposed to high concentrations of KCl (0.1-0.3 M) for periods of 4-5 hr, or to ethyl alcohol (5-30%) for periods of 2 hr. The treatment started at various phases of the free-running circadian rhythm in continuous light. Whereas the K(+) pulses induced only advance

[Effect of nitrogen, phosphorus and potassium in the production and nutritional value of beans (Phaseolus vulgaris L.)].

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Adhesion of Colletotrichum lindemuthianum Spores to Phaseolus vulgaris Hypocotyls and to Polystyrene.

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Adhesion of Colletotrichum lindemuthianum spores to Phaseolus vulgaris hypocotyls and to polystyrene was inhibited by the respiratory inhibitors sodium azide and antimycin A, indicating a requirement for metabolic activity in adhesion. Various commercial proteins and Tween 80 also reduced adhesion

Essential Elements in Dry and Canned Kidney Beans ( Phaseolus vulgaris L.).

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Ten essential mineral elements were determined in dry and in canned kidney beans ( Phaseolus vulgaris L.). Samples were taken at different stages during the canning process to determine where changes in element content occurred. Canned kidney beans contained significantly lower concentrations of

Rhizobium tropici genes involved in free-living salt tolerance are required for the establishment of efficient nitrogen-fixing symbiosis with Phaseolus vulgaris.

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Characterization of nine transposon-induced mutants of Rhizobium tropici with decreased salt tolerance (DST) allowed the identification of eight gene loci required for adaptation to high external NaCl. Most of the genes also were involved in adaptation to hyperosmotic media and were required to

Determination of seed-born percentages of bean common mosaic necrosis virus (BCMNV) in three genotypes of Phaseolus vulgaris.

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Bean common mosaic necrosis virus (BCMNV) is one of the most damaging viruses of bean that naturally transmitted by aphid in non persistent manner and through the seeds. BCMNV belongs to the genus Potyvirus and the family Potyviridae. During the growing season of 2004, bean leaf samples with viral

Stability of tobacco-mosaic virus, Marmor tabaci H, in solutions diluted beyond the end point of infectivity.

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Thornberry, H. H. (University of Illinois, Urbana, Ill.) and B. B. Nagaich. Stability of tobacco-mosaic virus, Marmor tabaci H, in solutions diluted beyond the end point of infectivity. J. Bacteriol. 83:1322-1326. 1962.-Tobacco-mosaic virus (TMV) in crude plant extract and in a partially purified

Calcium Deficiency of Dark-grown Seedlings of Phaseolus vulgaris L.

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Hypocotyl collapse in dark-grown seedlings of Phaseolus vulgaris cv. Pinto was due to calcium deficiency. There was no evidence of an associated pathogen. The number of seedlings with hypocotyl collapse decreased and the mean hypocotyl length increased when increasing levels of calcium (0-100

Effect of processing on the nutrients and anti-nutrients of lima bean (Phaseolus lunatus L.) flour.

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The effect of soaking, autoclaving and toasting methods on the proximate and mineral composition and anti-nutrients of lima (butter) bean (Phaseolus lunatus L.) was investigated. The raw seed showed high content of proteins, lipids and ash with good values of K, Ca, Mg, P, Na and Fe in comparison

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