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Ribonuclease Activity Associated With Ribosomes of Zea mays.

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At pH 6.5, a ribonuclease(s) is associated with ribosomes isolated from corn (Zea mays L.) and cannot be removed by repeated differential centrifugation or by sedimenting through the sucrose gradient. The enzyme is active under conditions favoring the maintenance of integrity of the ribosomes.

Effects of Potassium Nutrition on Ribonucleic Acid and Ribonuclease in Zea mays L.

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Potassium deficiency decreased the total RNA per shoot in corn (Zea mays L.) seedlings due to the reduced sizes of the plant, but increased the ratio of RNA to dry matter as much as 40%. Total base composition of RNA was unaffected by K(+) deficiency. Thus K(+) deficiency does not appear to alter

Ribonuclease Levels in the Mesocotyl Tissue of Zea mays as a Function of 2,4-Dichlorophenoxyacetic Acid Application.

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A ribonuclease III domain protein functions in group II intron splicing in maize chloroplasts.

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Chloroplast genomes in land plants harbor approximately 20 group II introns. Genetic approaches have identified proteins involved in the splicing of many of these introns, but the proteins identified to date cannot account for the large size of intron ribonucleoprotein complexes and are not

Granular bodies in root primary meristem cells of Zea mays L. var. Cuscoensis K. (Poaceae) that enter young vacuoles by invagination: a novel ribophagy mechanism.

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Because it has a very large, very rapidly growing primary root, we evaluated giant maize (Zea mays var. Cuscoensis) as a model organism for root research. Granular inclusions are a common feature of cells in many organisms, but they are not common in root meristems. We here report the presence of

Plant Nucleases: V. Survey of Corn Ribonuclease II Isoenzymes.

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Corn (Zea mays L.) ribonuclease II of the root microsomal fraction was isolated from 11 inbreds and seven hybrids. Polyacrylamide gel electrophoresis revealed a total of five bands of activity among corn lines tested. Most inbreds had only one isoenzyme, but three had two isoenzymes. The hybrids

Ribonuclease Activity in Normal, opaque-2, and floury-2 Maize Endosperm during Development.

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The elevated ribonuclease activity produced in the endosperm of a maize (Zea mays L.) inbred, W64A, by homozygous opaque-2, results from a more than doubled rate of ribonuclease accumulation occurring prior to 16 days post-pollination; after 16 days the rates in opaque-2 and normal are the same,

Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water Stress.

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Sucrose gradient profiles of polyribosomes from the coleoptilar node region of seedlings of Zea mays L. were obtained without pelleting and redispersion of the particles. Water stress caused a shift of ribosomes from the polymeric to the monomeric form, starting about 30 minutes after stress

Characterization of ribonucleic acid synthesis by nuclei isolated from Zea mays.

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Nuclei were isolated from the shoots of Zea mays and assayed for endogenous RNA polymerase activity in vitro. Maximum incorporation from radioactive precursors (70 pmol [(3)H]uridine 5' monophosphate/100 μg DNA) was reached after incubation for 1 h at 25°C. The RNA product, analysed by

Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA

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Ustilago maydis genome codes for many secreted ribonucleases. The contribution of two among these belonging to the T2 family (Nuc1 and Nuc2) in the pathogen virulence, has been assessed in this study. The nuc1 and nuc2 deletion mutants showed not only reduced pathogenicity compared to the SG200 WT

The Zea mays glycine-rich RNA-binding protein MA16 is bound to a ribonucleotide(s) by a stable linkage.

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Expression of the gene encoding the maize glycine-rich RNA-binding protein MA16 is developmentally regulated and it is involved in environmental stress responses. The MA16 protein shows a wide spectrum of RNA-binding activities. On the basis of in vivo labelling, where a [³²P]phosphate label was

Cytochemical studies concerning the occurrence and distribution of RNA in plastids of Zea mays.

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The occurrence of RNA in plastids from etiolated and green maize leaves was demonstrated cytochemically, with both the light and the electron microscope. Etiolated leaves were allowed to incorporate tritiated cytidine for several hours and were subsequently fixed in formalin. Radioautographs of leaf

Nitrate Reductase Activity and Polyribosomal Content of Corn (Zea mays L.) Having Low Leaf Water Potentials.

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Desiccation of 8- to 13-day-old seedlings, achieved by withholding nutrient solution from the vermiculite root medium, caused a reduction in nitrate reductase activity of the leaf tissue. Activity declined when leaf water potentials decreased below -2 bars and was 25% of the control at a leaf water

Calorie restriction decreases platelet-derived growth factor (PDGF)-A and thrombin receptor mRNA expression in autoimmune murine lupus nephritis.

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Calorie restriction (CR) and supplementation with fish oil (FO) are known to increase the life span and diminish histological evidence of glomerulonephritis in lupus prone (NZB x NZW)F1 (B/W) mice. Cellular proliferation is an important pathological element in the development of lupus nephritis, and

Evolution of retroposons by acquisition or deletion of retrovirus-like genes.

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The retroid family consists of all genetic elements that encode a potential reverse transcriptase (RT). Members of this family include a diversity of eukaryotic genetic elements (viruses, transposable elements, organelle introns, and plasmids) and the retrons of prokaryotes. Some retroid elements

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