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The Role of Weed Hosts and Tobacco Thrips, Frankliniella fusca, in the Epidemiology of Tomato spotted wilt virus.

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Wild plant species were systematically sampled to characterize reproduction of thrips, the vector of Tomato spotted wilt virus (TSWV), and natural sources TSWV infection. Thrips populations were monitored on 28 common perennial, biennial, and annual plant species over two noncrop seasons at six

Size and complexity of polyadenylated RNAs induced in tobacco infected with sonchus yellow net virus.

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Hybridization of 32P-labeled sonchus yellow net virus (SYNV) RNA to polyadenylated (poly A+) RNA from infected tobacco reveals the presence of four electrophoretically distinct components. These components probably represent five discrete RNA species complementary to SYNV RNA (scRNAs). The scRNAs

Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polysomes of infected tobacco.

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Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral

Ageratum enation virus-a begomovirus of weeds with the potential to infect crops.

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Samples of two Ageratum conyzoides, one Sonchus oleraceus and one turnip (Brassica rapa var. rapa) exhibiting virus-like symptoms were collected from Pakistan and Nepal. Full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. The

First Report of Olive mild mosaic virus and Sowbane mosaic virus in Spinach in Greece.

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Uncommon, viruslike symptoms (yellowing, line patterns, leaf deformation, and necrosis), were observed in spinach fields in the Marathon area, Greece in 2004. Seedlings from the same seed lot, grown in the greenhouse, also developed the same viruslike symptoms, indicating that the causal agent(s) of

First Report of Natural Infection of Greenhouse-Grown Tomato and Weed Species by Pelargonium zonate spot virus in Spain.

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Tomato (Lycopersicon esculentum Mill.) plants showing severe chlorotic and necrotic ringspots, line patterns on leaves, and concentric chlorotic ringspots on stems and fruits were observed in plastic greenhouse-grown tomato crops cv. Royesta during the spring of 1996 in Zaragoza province, Northeast

Natural Infection of Citrullus colocynthis by Papaya ringspot virus-W in Iran.

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Papaya ringspot virus (PRSV, genus Potyvirus, family Potyviridae) is economically important due to its worldwide distribution and because it can cause serious losses in both cucurbit crops and papaya (3). PRSV has been previously reported from cucurbit crops in Iran (2). In Khuzestan Province,

Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia.

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A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA

First Report of Tomato mosaic virus on Common Sow Thistle in Iran.

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The natural incidence of Tomato mosaic virus (ToMV) in common sow thistle (Sonchus oleraceus) from vegetable fields was assessed to determine the role of this weed species as a virus inoculum source. Twenty sow thistle plants with virus-like foliar symptoms including mosaic and malformations were

Structure of the nucleocapsid protein gene of sonchus yellow net virus.

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The structure of the gene adjacent to the "leader RNA" gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, was deduced by dideoxyribonucleotide sequence analysis of SYNV genomic (g) RNA and a series of plasmids constructed from SYNV gRNA or polyadenylated [poly(A)+] RNA from SYNV-infected

Structure of the M2 protein gene of sonchus yellow net virus.

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The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this

Detection and sequence of plus-strand leader RNA of sonchus yellow net virus, a plant rhabdovirus.

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Tobacco infected with the plant rhabdovirus sonchus yellow net virus (SYNV) contains short, 139- to 144-nucleotide (nt) transcripts complementary to the 3' terminus of the negative-strand genomic RNA. These transcripts are similar to the leader RNAs associated with several animal rhabdovirus

Subcellular distribution of RNA sequences complementary to sonchus yellow net virus RNA.

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RNA isolated from free and membrane-bound polyribosomes of sonchus yellow net virus (SYNV)-infected tobacco was hybridized to SYNV RNA. RNA from free polyribosomes was shown to be complementary to nearly 100% of the SYNV genome, whereas RNA from membrane-bound polyribosomes was complementary to only

Replication of sonchus yellow net virus in infected protoplasts.

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Tobacco (Nicotiana edwardsonii and N. benthamiana) protoplasts infected with the plant rhabdovirus sonchus yellow net virus (SYNV) were found to be suitable for studies of replication. SYNV messenger RNAs could be detected within 2 hr postinoculation (PI), accumulated to a maximum within 24 hr, and

Construction of a Sonchus Yellow Net Virus minireplicon: a step toward reverse genetic analysis of plant negative-strand RNA viruses.

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Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR

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