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Succinate dehydrogenase : a partial purification from mung bean hypocotyls and soybean cotyledons.

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A procedure was developed for the partial purification of succinate dehydrogenase from mung bean (Vigna radiata L.) hypocotyls and soybean (Glycine max [L] Merr. v. Ransom) cotyledons. The procedure utilized a Triton X-100 extraction followed by ammonium sulfate precipitation. The final fraction was

Alternative Oxidase Activity and the Ubiquinone Redox Level in Soybean Cotyledon and Arum Spadix Mitochondria during NADH and Succinate Oxidation.

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In Arum and soybean (Glycine max L.) mitochondria, the dependence of the alternative oxidase activity on the redox level of ubiquinone, with NADH and succinate as substrates, was studied, using a voltametric procedure to measure the ubiquinone redox poise in the mitochondrial membrane. The results

Nitrate Reductase Activity in Soybeans (Glycine max [L.] Merr.): II. Energy Limitations.

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Growth chamber studies with soybeans (Glycine max [L.] Merr.) were designed to determine the relative limitations of NO(3) (-), NADH, and nitrate reductase (NR) per se on nitrate metabolism as affected by light and temperature. Three NR enzyme assays (+NO(3) (-)in vivo, -NO(3) (-)in vivo, and in

Cytokinin inhibition of respiration by cells and mitochondria of soybean, Glycine max (L.) Merrill.

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Cells of a soybean tissue strain, suspended in an aerated liquid medium, caused disappearance of p-coumaric acid from the medium and oxidation of guaiacol, benzidine, pyrogallol, L-dihydroxyphenylalanine and L-epinephrine. Both the disappearance and the oxidations were inhibited by

Effects of exogenous bisphenol A on the function of mitochondria in root cells of soybean (Glycine max L.) seedlings.

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Bisphenol A (BPA), a contaminant of emerging concern, can affect plant root growth by changing various physiological processes. Mitochondria are critical organelles that produce energy for growth. However, how BPA affects the function and ultrastructure of mitochondria and then plant root growth

Cytokinin inhibition of respiration in mitochondria from six plant species.

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The influence of 6-benzylaminopurine (BAP) on the respiration by mitochondria from bush bean (Phaseolus vulgaris L.), mung bean (P. aureus Roxburgh), soybean [Glycine max (L.) Merrill], maize (Zea mays L.), pea (Pisum sativum L.), and wheat (Triticum aestivum L.) was examined. BAP, a synthetic

Purification, identification, and characterization of peanut isocitrate lyase.

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Isocitrate lyase (ICL, EC 4.1.3.1) is commonly present in oil-rich seeds in catalyzing the cleavage of isocitrate to glyoxylate and succinate and plays an essential role in lipid metabolism and gluconeogenesis. When peanut kernels (Tainan 14) were germinated at 30 degrees C, the cotyledon ICL

Effect of protein additives on acetylene reduction (nitrogen fixation) by Rhizobium in the presence and absence of soybean cells.

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The effect of protein additives on acetylene reduction (N(2) fixation) by Rhizobium associated with soybean cells (Glycine max [L.] Merr.) in vitro was studied. Acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of N/ml supplied as aqueous extracts of hexane-extracted

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons.

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The subcellular localization of enzymes involved in the 4-ami-nobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to

Regulation of Alternative Oxidase Activity by Pyruvate in Soybean Mitochondria.

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The regulation of alternative oxidase activity by the effector pyruvate was investigated in soybean (Glycine max L.) mitochondria using developmental changes in roots and cotyledons to vary the respiratory capacity of the mitochondria. Rates of cyanide-insensitive oxygen uptake by soybean root

Regulation of Alternative Pathway Activity in Plant Mitochondria : Deviations from Q-Pool Behavior during Oxidation of NADH and Quinols.

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External NADH and succinate were oxidized at similar rates by soybean (Glycine max) cotyledon and leaf mitochondria when the cytochrome chain was operating, but the rate of NADH oxidation via the alternative oxidase was only half that of succinate. However, measurements of the redox poise of the

Oxidative and phosphorylative activities of mitochondria isolated from cotton hypocotyls.

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Mitochondria isolated from 2 strains of cotton plant hypocotyls (Gossypium hirsutum L. var. Rex smooth leaf and Rex glandless) were examined for their oxidative phosphorylation activities. Bovine serum albumin at a relatively high concentration was essential in the extraction medium for the

Direct Inhibition of Plant Mitochondrial Respiration by Elevated CO2.

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Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine

Electron Partitioning between the Cytochrome and Alternative Pathways in Plant Mitochondria.

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The contribution of the cyanide-resistant, alternative pathway to plant mitochondrial electron transport has been studied using a modified aqueous phase on-line mass spectrometry-gas chromatography system. This technique permits direct measurement of the partitioning of electrons between the

Effect of nitrate on the organic Acid and amino Acid composition of legume nodules.

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Nitrate supplied to legume plants inhibits the activity of nitrogenase in Rhizobium bacteroids in root nodules. The accumulation of amino N which is known to occur in Glycine max (L.) Merr. nodules as nitrogenase activity declines was studied in more detail by analysis of changes in free amino acid

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