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Stability of live attenuated classical swine fever cell culture vaccine virus in liquid form for developing an oral vaccine

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Classical swine fever (CSF) is an important viral disease of pigs and controlled by vaccination. Unorganised backyard and wild pigs are difficult to vaccinate by needle vaccination. Here we formulated liquid vaccines using an Indian CSF cell culture vaccine virus and four stabilisers and evaluated

Phase I study of iv mycobacterial cell wall skeleton and cell wall skeleton combined with trehalose dimycolate.

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A phase I study to determine the toxicity and immunologic effects of iv administered aqueous suspensions of mycobacterial cell wall skeleton (CWS) and CWS combined with trehalose dimycolate (TDM) was performed. A total of 26 patients received suspensions of either CWS or CWS/TDM given iv every 1 or

Discovery of Salmonella trehalose phospholipids reveals functional convergence with mycobacteria.

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Salmonella species are among the world's most prevalent pathogens. Because the cell wall interfaces with the host, we designed a lipidomics approach to reveal pathogen-specific cell wall compounds. Among the molecules differentially expressed between Salmonella Paratyphi and S.

Phase I study of intravenous mycobacterial cell wall skeleton and trehalose dimycolate attached to oil droplets.

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The toxic, clinical, and immunological effects of suspensions of mycobacterial cell wall skeleton (CWS) and trehalose dimycolate (TDM) attached to oil droplets and given intravenously in doses of 100-2,000 micrograms/m2 (CWS) every 1 or 2 weeks was investigated in this Phase I study. The major

Potency of a thermostabilised chimpanzee adenovirus Rift Valley Fever vaccine in cattle.

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Development of safe and efficacious vaccines whose potency is unaffected by long-term storage at ambient temperature would obviate major vaccine deployment hurdles and limit wastage associated with breaks in the vaccine cold chain. Here, we evaluated the immunogenicity of a novel chimpanzee

Administration of BCG cell wall skeleton into malignant effusions: toxic and therapeutic effects.

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Thirty-nine patients with 40 refractory malignant effusions (26 pleural and 14 peritoneal) were treated locally with a nonviable mycobacterial vaccine. The vaccine was administered into the effusion and consisted of BCG cell wall skeleton and trehalose dimycolate attached to oil microdroplets. A

"IDEAL" vaccines for resource poor settings.

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In developing countries, immunization delivery would be more efficient, safer and economical if all vaccines could elicit long-term protection following needle-free administration of just a single dose and without need for a cold chain, and if immunization left an indelible (e.g., serologic) marker

[Adaptation of Flammulina velutipes to hypothermia in natural environment: the role of lipids and carbohydrates].

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The role of lipids and carbohydrates in the adaptation of Flammulina velutipes to hyperthermia (5 to -5 degrees C) in natural environments has been studied for the first time. The main changes are were found to occur in membrane lipids: the levels of sterols and glycolipids decreased, and the

[About different mechanisms of the biochemical adaptation of mycelial fungi to temperature stress: changes in cytosol carbohydrate composition].

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The effect of temperature stress on the cytosol carbohydrate composition of fungi belonging to various systematic groups was investigated. In Mucorales representatives (subkingdom Eomycota, phylum Archemycota, class Zygomycetes), adaptation to hypo- and hyperthermia occurs via the regulation of

Clinical immunotherapy trials of bacterial components derived from Mycobacteria and Nocardia.

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Preparations of oil-attached mycobacterial components have been used in place of viable bacille Calmette-Guérin (BCG) in animal models and humans as a cancer immunotherapeutic agent. Most preparations consist of the isolated mycobacterial cell wall or cell wall skeleton attached to oil. Cord factor

Clinical Phase I investigation of intravenous oil attached mycobacterial components as immunotherapeutic agents.

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The toxicity and immunological effects of suspensions of mycobacterial cell wall skeleton (CWS) and trehalose dimycolate (TDM) attached to oil droplets and given intravenously in doses of 100 to 200 micrograms/M2 every one or two weeks was investigated. The major limiting side effect was fever and

Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.

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Recombinant virus-like particles (VLP) antigenically similar to rabbit hemorrhagic disease virus (RHDV) were recently expressed at high levels inside Pichia pastoris cells. Based on the potential of RHDV VLP as platform for diverse vaccination purposes we undertook the design, development and

Isolation of a nontoxic lipid A fraction containing tumor regression activity.

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Galanos-type endotoxin obtained from the heptose-less mutant of Salmonella typhimurium was converted to Lipid A by two cycles of treatment with sodium acetate, pH 4.5, at 100 degrees and separated on a DEAE-cellulose column into several fractions (Fractions III to VII). Tumor regression studies with

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