Fast correction of B0 field inhomogeneity for pH-specific magnetization transfer and relaxation normalized amide proton transfer imaging of acute ischemic stroke without Z-spectrum.

The magnetization transfer and relaxation normalized amide proton transfer (MRAPT) analysis is promising to provide a highly pH-specific mapping of tissue acidosis, complementing commonly used CEST asymmetry analysis. We aimed to develop a fast B₀ inhomogeneity correction algorithm for acute stroke magnetization transfer and relaxation normalized amide proton transfer imaging without Z-spectral interpolation.

The proposed fast field inhomogeneity correction describes B₀ artifacts with linear regression. We compared the new algorithm with the routine interpolation correction approach in CEST imaging of a dual-pH phantom. The fast B₀ correction was further evaluated in amide proton transfer imaging of normal and acute stroke rats.

Our phantom data showed that the proposed fast B₀ inhomogeneity correction significantly improved pH MRI contrast, recovering over 80% of the pH MRI contrast-to-noise-ratio difference between the raw magnetization transfer ratio asymmetry and that using the routine interpolation-based B₀ correction approach. In normal rat brains, the proposed fast B₀ correction improved pH-specific MRI uniformity across the intact tissue, with the ratio of magnetization transfer and relaxation normalized amide proton transfer ratio being 10% of that without B₀ inhomogeneity correction. In acute stroke rats, fast B₀ inhomogeneity-corrected pH MRI reveals substantially improved pH lesion conspicuity, particularly in regions with nonnegligible B₀ inhomogeneity. The pH MRI contrast-to-noise ratio between the ipsilateral diffusion lesion and contralateral normal tissue improved significantly with fast B₀ correction (from 1.88 ± 0.48 to 2.20 ± 0.44, P < .01).

Our study established an expedient B₀ inhomogeneity correction algorithm for fast pH imaging of acute ischemia.