The effect of Codonopis bulleynana Forest ex Diels on chronically constipated mice.

To verify the laxative effect of Codonopsis bulleyana and its effect on intestinal microbiota, a long-term constipation model was established using 3.0 mg/kg loperamide hydrochloride, after which, the long-term constipation model was administered by 0.2 g/ml high-dose Codonopsis bulleyana water extract. The therapeutic effects were observed by measuring defecation amount and feces moisture content. The composition of intestinal microbiota was detected and analyzed using16S rDNA sequencing technology. The results showed that Codonopsis bulleyana water extract can increase stool quantity and promote intestinal tract movement in constipated mice. Obvious changes were shown in intestinal microbiota of chronically constipated mice treated with Codonopsis bulleyana water extract as the proportion of beneficial bacteria increased in the model treated by Codonopsis bulleyana. Codonopsis bulleyana water extract alleviates constipation symptoms caused by loperamide hydrochloride and improves the intestinal microbiota in constipated mice.