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Promotion of crystalline cellulose degradation by expansins from Oryza sativa.

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CONCLUSIONS Enzymatic activities of Oryza sativa expansins, which were heterologously overexpressed in Escherichia coli , were analyzed. Results suggested that expansins promote degradation of cellulose by cellulase in a synergistic manner. Sustainable production of future biofuels is dependent on

Isolation and in vitro fusion of egg and sperm cells in Oryza sativa.

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Protocols for isolation of gametes of Oryza sativa were developed and initial results on in vitro fusion of sperm and egg cells are reported. The best yield of viable sperm cells was obtained when pollen grains were cultured in a medium containing of 1.3 mM boric acid, 3.6 mM calcium chloride, 0.74

Analysis of the Oryza sativa plasma membrane proteome using combined protein and peptide fractionation approaches in conjunction with mass spectrometry.

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To identify integral and peripheral plasma membrane (PM) proteins from Oryza sativa (rice), highly enriched PM fractions from rice suspension cultured cells were analyzed using two complementary approaches. The PM was enriched using aqueous two-phase partitioning and high pH carbonate washing to

An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen

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Rice is an important food crop in the world, and rice blast is one of the major fungal diseases that cause large-scale rice yield reduction. This study aimed to investigate the antagonistic activity and its molecular mechanism of bacterial strain JK isolated from seeds of super hybrid rice

Ethanol production from Rice (Oryza sativa) straw by simultaneous saccharification and cofermentation.

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Ethanol production from alkali treated rice straw was investigated by simultaneous saccharification and cofermentation (SSCF) using commercial cellulase and 3 different yeast strains viz., Saccharomyces cerevisiae HAU-1, Pachysolen tannophilus and Candida sp. individually as well as in combination

Functional characterization of antagonistic fluorescent pseudomonads associated with rhizospheric soil of rice (Oryza sativa L.).

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Antagonistic fluorescent pseudomonads isolated from rhizospheric soil of rice were characterized by 16S rRNA amplicon and fatty acid methyl ester (FAME) analyses. Antagonistic isolates were grown in the fermentation media, and production of antibiotics was confirmed by thin-layer chromatography

Bioethanol production from dedicated energy crops and residues in Arkansas, USA.

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Globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. Lignocellulosic biomass of four dedicated energy crops [giant reed (Arundo donax L.), elephantgrass (Pennisetum purpureum (Schumach), Miscanthus × giganteus (Illinois

Comparison of saccharification and fermentation of steam exploded rice straw and rice husk.

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BACKGROUND Rice cultivation produces two waste streams, straw and husk, which could be exploited more effectively. Chemical pretreatment studies using rice residues have largely focussed on straw exploitation alone, and often at low substrate concentrations. Moreover, it is currently not known how

LSSR1 facilitates seed setting rate by promoting fertilization in rice.

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Seed setting rate is one of the major components that determine rice (Oryza sativa L.) yield. Successful fertilization is necessary for normal seed setting. However, little is known about the molecular mechanisms governing this process. In this study, we report a novel rice gene, LOW SEED SETTING

[Optimized condition for protoplast isolation from maize, wheat and rice leaves].

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Maize (Zea mays L.), wheat (Triticum aestivum L.) and rice (Oryza sativa L.) are three staple crops and accordingly it is very meaningful to optimize the condition of their protoplasts isolation. The concentration of the enzyme, the time of isolation and centrifugal force in protoplast isolation

Endo-(1,4)-β-glucanase gene families in the grasses: temporal and spatial co-transcription of orthologous genes.

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BACKGROUND Endo-(1,4)-β-glucanase (cellulase) glycosyl hydrolase GH9 enzymes have been implicated in several aspects of cell wall metabolism in higher plants, including cellulose biosynthesis and degradation, modification of other wall polysaccharides that contain contiguous (1,4)-β-glucosyl

Isolation of nuclei from rice tissue grown in suspension culture.

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A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA

Quality determination of nickel-loaded silica prepared from poaceous biomass.

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Klason lignin or preacid hydrolysate of a poaceous biomass such as rice husk, rice straw ( Oryza sativa ), and wheat straw ( Triticum aestivum ) became a good source of highly pure silica by simple calcinations in the testing process for application of high-boiling solvent (HBS) pulping of

Evidence for the involvement of glycanase activities in the dissociation of cortical cell walls during the emergence of callus from rice root tissues in the presence of 2,4-D.

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Nojirimycin bisulfite andp-nitrophenyl-1-thio-ß-D-glucoside, potent inhibitors of ß-glucanase, suppressed cortical dissociation and callus growth in rice roots (Oryza sativa L. cv. Sasanishiki) in the presence of 2,4-D, with resultant suppression of the emergence of callus clumps from the interior

Expression of an Acidothermus cellulolyticus endoglucanase in transgenic rice seeds.

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The thermostable endo-1,4-β-glucanase (E1) from Acidothermus cellulolyticus, is a useful enzyme for commercial hydrolysis of cellulose into glucose. A codon-optimized synthetic gene encoding this enzyme was transformed into rice (Oryza sativa L. ssp. japonica) under the control of the rice seed

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