concanavalin a/кариес зубов

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Increased clearance of Candida albicans from the peritoneal cavity of mice pretreated with concanavalin A or jacalin.

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We have studied the role of polymorphonuclear leukocytes and macrophages in the clearance of Candida albicans from the peritoneal cavity of Swiss mice after treatment with jacalin or concanavalin A (Con-A). Mice (25-30 g, N = 7 per group) received jacalin or Con-A (500 micrograms/0.5 ml PBS)

Magnetic resonance studies of concanavalin A: location of the binding site of alpha-methyl-D-mannopyranoside.

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The longitudinal nuclear magnetic relaxation times of the methyl protons of alpha-methyl-D-mannopyranoside have been measured at 90 MHz and 270 MHz in solutions of concanavalin A complexed with: (i) Mn2+ and Ca2+; and (ii) Zn2+ and Ca2+. Zn2+ and Mn2+ are known to bind in site S1 and Ca2+ in site S2

Initial microbial colonization of enamel in children with different levels of caries activity: An in situ study.

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OBJECTIVE To investigate patterns of overnight in situ microbial colonization of enamel in children. METHODS Overall, 29 children (aged 5-9 years) participated in the study. Nine were caries-free with no decayed, missing, or filled teeth (DMFT), 11 were caries-rehabilitated (DMFT ≥ 2, no active

A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane.

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A continuous glucose monitoring device that resides fully in the subcutaneous tissue has the potential to greatly improve the management of diabetes. Toward this goal, we have developed a competitive binding glucose sensing assay based on fluorescently labeled PEGylated concanavalin-A

Low-temperature water reconstruction in concanavalin A, with implications for controlled protein crystal annealing.

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Flash-cooled crystals of the I222 form of concanavalin A undergo a sharp non-destructive non-reversible phase transition upon warming to between 160 and 165 K, characterized by an anomalous increase in unit-cell volume. The expansion is anisotropic and primarily affects the b and c axes. Three sets

Concanavalin A application to the olfactory epithelium reveals different sensory neuron populations for the odour pair D- and L-carvone.

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Carvone enantiomers (D and L optical isomers) have been shown to be discriminable by humans even though the odor qualities are quite similar. Our experiment is based on a finding (J. Steroid Biochem. Molec. Biol. 1991;39(4B):621) that Concanavalin A (ConA) applied to a frog olfactory epithelium

Genetic control of lymphocyte activation: lack of response to low doses of concanavalin A in lipopolysaccharide-nonresponder mice.

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C3H/HeJ mice do not respond to the polyclonal B-cell activator lipopolysaccharide (LPS) from Escherichia coli; this was first described by Sultzer who observed that mice of this strain did not respond to an intraperitoneal (i.p.) injection of LPS as measured by the accumulation of leukocytes in the

The covalent and three-dimensional structure of concanavalin A. II. Amino acid sequence of cyanogen bromide fragment F3.

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The amino acid sequence of the COOH-terminal CNBr fragment, F3 (residues 130 to 237), of concanavalin A has been established, completing the determination of the covalent structure of this lectin. Analysis of the chemical sequence showed that the distribution of charged residues is generally more

Concanavalin A-induced activation of hamster mast cells: morphological changes and histamine secretion.

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Peritoneal mast cells of Syrian hamsters release histamine to the action of concanavalin A (Con A) in dose-dependent fashion. The rate of release was very rapid in the first seconds of cell activation and completed in 60 s after the challenge. Morphological changes concomitant to the lectin

Enzymology and Ultrastructure of the in situ Pellicle in Caries-Active and Caries-Inactive Patients.

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The present study aimed to evaluate the impact of caries activity on the key enzymes and the ultrastructure of the in situ pellicle. Pellicle formation was performed on bovine enamel slabs. Intraoral exposure (3, 30, and 120 min) was accomplished by 14 caries-active (DMFS: 22.7 ± 12.1) and 13

Effective immunity to dental caries: enhancement of salivary anti-Streptococcus mutans antibody responses with oral adjuvants.

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In the present study, we compared the ability of the soluble adjuvants concanavalin A (ConA), muramyl dipeptide (MDP), and peptidoglycan (PG) to enhance immune responses to orally administered particulate antigens of Streptococcus mutans 6715 in gnotobiotic rats. The isotype and levels of antibody

Heterogeneity of concanavalin A binding by mouse peritoneal macrophages.

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The binding of the lectin concanavalin A (Con A) to the cell surface of monocytes and macrophages collected from the stimulated peritoneal cavity of mice was investigated electron microscopically with horseradish peroxidase-gold as an indirect marker. Individual cells were identified by the

Embryonal carcinoma in the abdominal cavity of a male calf.

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An embryonal carcinoma was diagnosed in the abdominal cavity of a 55-day-old male calf. Macroscopically, a large volume of ascitic fluid was observed along with white to yellowish-white masses of various sizes densely located on the abdominal wall and the surface of abdominal organs. There was an

Inhibition of the accumulation of macrophages and the generation of macrophage chemotactic activity by dexamethasone in concanavalin A-induced peritonitis of mice.

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A delayed-type inflammatory response was evoked in mice using concanavalin A (Con A) as a stimulus, and the effect of various anti-inflammatory agents on the inflammation was examined. The intraperitoneal injection of Con A in the mouse resulted in the marked accumulation of leukocytes, especially

Binding and functional properties of concanavalin A and its derivatives. III. Interactions with indoleacetic acid and other hydrophobic ligands.

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The binding of concanavalin A to various structures via hydrophobic interactions has been studied using a variety of physicochemical assays. It was found that concanavalin A binds to nonpolar compounds such as the plant auxin beta-indoleacetic acid and its structural analogue tryptophan and that

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