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Translation of specific vaccinia virus RNAs purified as RNA-DNA hybrids on potassium iodide gradients.

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A procedure has been developed for purifying specific mRNAs by hybridization to fragments of DNA and isolation of the hybrids by potassium iodide equilibrium buoyant density centrifugation. The hybrids obtained are essentially free of unhybridized RNA as well as double-stranded RNA. Moreover, the

Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus.

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A recombinant vaccinia virus containing a Drosophila potassium channel (Shaker H4) cDNA was constructed by homologous recombination between wild-type vaccinia virus DNA and a transfer plasmid. The new virus was used to infect four types of mammalian cells in culture. Electrophysiological recording

Human homologue of the Drosophila discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes.

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Human homologue of the Drosophila discs large tumor suppressor protein (hDlg) belongs to a newly discovered family of proteins termed MAGUKs that appear to have structural as well as signaling functions. Consistent with the multi-domain organization of MAGUKs, hDlg consists of three copies of the

Protein kinase activity from vaccinia virions: solubilization and separation into heat-labile and heat-stable components.

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A protein kinase was solubilized from whole vaccinia virions by using a solution containing deoxycholate, dithiothreitol, and sodium or potassium chloride. The released enzyme was completely dependent on Mg(2+) and was greatly stimulated by added basic proteins such as protamine or histones.

Identification of the feline herpesvirus type 1 (FHV-1) genes encoding glycoproteins G, D, I and E: expression of FHV-1 glycoprotein D in vaccinia and raccoon poxviruses.

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The genome of feline herpesvirus type 1 (FHV-1), the major cause of viral upper respiratory disease in cats, contains several genes encoding homologues of herpes simplex virus type 1 (HSV-1) glycoproteins. Restriction mapping studies have indicated that the group D genome of FHV-1 contains a unique

Protein kinase and specific phosphate acceptor proteins associated with vaccinia virus cores.

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Incubation of purified vaccinia virus with gamma-(32)P-adenosine triphosphate resulted in the incorporation of (32)P into hot trichloroacetic acid-insoluble material. Enzymatic activity was completely dependent on the addition of divalent cations and was stimulated by nonionic detergents and

Molecular weight of Yaba monkey tumor virus DNA.

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The molecular weight of Yaba virus DNA was determined by the isolation of intact DNA genomes from Yaba virus, which had been purified by two sucrose density gradient and one potassium tartrate gradient centrifugations, by cosedimentation with T2 DNA. The molecular weight of Yaba DNA was calculated

Covalent DNA binding by vaccinia topoisomerase results in unpairing of the thymine base 5' of the scissile bond.

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We have used potassium permanganate to probe contacts between vaccinia DNA topoisomerase and thymine residues in its 5'-CCCTT downward arrow DNA target site. Two major conclusions emerge from the experiments presented: (i) permanganate oxidation of the +2T base of the scissile strand interferes with

Spectrum of physical properties among the virions of a whole population of vaccinia virus particles.

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Populations of virions released from cultures of L cells infected with vaccinia virus are composed of particles which differ substantially from each other in sedimentation rate and buoyant density. Clumps of two and three virions sediment enough faster than single particles so that fractions

CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : IV. DEMONSTRATION OF COPPER IN THE PURIFIED VIRUS.

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A search by means of spectroscopic and enzymatic techniques has failed to demonstrate either cytochrome or cytochrome oxidase in purified elementary bodies of vaccinia. A constituent of the virus which catalyzes the oxidation of cystein has been found and identified as copper in a concentration

5'-Terminal 7-methylguanosine and mRNA function: influence of potassium concentration on translation in vitro.

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Vaccinia mRNAs containing either 5'-terminal m7G or unmethylated 5'-terminal structures were synthesized in vitro and their relative efficiencies of translation were compared in wheat germ and reticulocyte cell-free protein-synthesizing systems. The importance of the m7G group for efficient

Synthesis and evaluation of some new 1,3,4-oxadiazoles bearing thiophene, thiazole, coumarin, pyridine and pyridazine derivatives as antiviral agents.

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In an attempt to produce heterocyclic compounds based on 1,3,4-oxadiazole derivatives with potential antiviral activity, synthesis of compound 1 [2-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)acetonitrile] was performed through the reaction of cyanoacetic acid hydrazide with carbon disulfide in

Phosphorylation and modulation of brain glutamate transporters by protein kinase C.

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High affinity sodium- and potassium-coupled L-glutamate transport into presynaptic nerve terminals and fine glial processes removes the neurotransmitter from the synaptic cleft, thereby terminating glutamergic transmission. This report describes that the purified L-glutamate transporter from pig

Immunological characterization of the feline herpesvirus-1 glycoprotein B and analysis of its deduced amino acid sequence.

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Feline herpesvirus 1 (FHV-1) is an important viral pathogen of cats. Like other alphaherpesviruses, FHV-1 contains a HSV-1 glycoprotein B (gB) homolog. In this study, monospecific antisera to HSV-1 gB reacted with three FHV-1 proteins (100, 64, and 58 kDa) present in virion lysates by

Effect of methylation of the N6 position of the penultimate adenosine of capped mRNA on ribosome binding.

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An RNA(2'-O-methyladenosine-N6)-methyltransferase isolated from HeLa cells was used to convert the ends of vaccinia virus mRNAs containing m7G(5')pppAm-to m7G(5')pppm6Am-. Under competitive conditions, there was no preferential binding of mRNAs containing m6Am residues within the capped ends

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