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Detection of hydrogen cyanide from oral anaerobes by cavity ring down spectroscopy.

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Hydrogen cyanide (HCN) has been recognized as a potential biomarker for non-invasive diagnosis of Pseudomonas aeruginosa infection in the lung. However, the oral cavity is a dominant production site for exhaled HCN and this contribution can mask the HCN generated in the lung. It is thus important to

Background levels of hydrogen cyanide in human breath measured by infrared cavity ring down spectroscopy.

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Hydrogen cyanide (HCN) in breath has been suggested as a diagnostic tool for cyanide poisoning and for cyanide-producing bacterial infections. To distinguish elevated levels of breath HCN, baseline data are needed. Background levels of HCN were measured in mixed exhaled air from 40 healthy subjects

Blastocoel cavity formation by preimplantation rat embryos in the presence of cyanide and other inhibitors of oxidative phosphorylation.

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The role of oxidative phosphorylation in blastocoel development in rats was determined by culturing morula stage embryos for 24 h in the presence of three inhibitors of ATP generation: cyanide, antimycin-A and 2,4-dinitrophenol (DNP). Rat morulae could form blastocysts in concentrations of cyanide

Three-dimensional solution structure of the cyanide adduct of a Met80Ala variant of Saccharomyces cerevisiae iso-1-cytochrome c. Identification of ligand-residue interactions in the distal heme cavity.

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The 1H NMR spectrum of the the cyanide adduct of a triply mutated Saccharomyces cerevisiae iso-1-cytochrome c (His39Gln/Met80Ala/Cys102Ser) in the oxidized form has been assigned through 1D NOE and 2D COSY, TOCSY, NOESY, and NOE-NOESY experiments; 562 protons out of a total of 683 have been

Cyanide binding and heme cavity conformational transitions in Drosophila melanogaster hexacoordinate hemoglobin.

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The reason for the presence of hemoglobin-like molecules in insects, such as Drosophila melanogaster, that live in fully aerobic environments has yet to be determined. Heme endogenous hexacoordination (where HisE7 and HisF8 axial ligands to the heme Fe atom are both provided by the protein) is a

Solution 1H, 15N NMR spectroscopic characterization of substrate-bound, cyanide-inhibited human heme oxygenase: water occupation of the distal cavity.

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A solution NMR spectroscopic study of the cyanide-inhibited, substrate-bound complex of uniformly (15)N-labeled human heme oxygenase, hHO, has led to characterization of the active site with respect to the nature and identity of strong hydrogen bonds and the occupation of ordered water molecules

The biohazard potential of cyanide poisoning during postmortem examination.

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A case of suicide by ingestion of cyanide salts provided an opportunity to determine whether personnel involved in postmortem examination of such cases are exposed to any significant degree of biohazard from cyanide remaining in body cavities or tissues of the decedent or both. It was found that

Recent developments in cyanide detection: a review.

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The extreme toxicity of cyanide and environmental concerns from its continued industrial use continue to generate interest in facile and sensitive methods for cyanide detection. In recent years, there is also additional recognition of HCN toxicity from smoke inhalation and potential use of cyanide

Metabolism of organonitriles to cyanide by rat nasal tissue enzymes.

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1. A method for determination of cyanide release during microsomal metabolism of organonitriles was developed. 2. Vmax values for cyanide release from acetonitrile, propionitrile, butyronitrile, isobutyronitrile, acrylonitrile, benzyl cyanide and succinonitrile were determined for rat nasal and

Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity.

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Chlorite dismutases (Clds) are heme b containing oxidoreductases that convert chlorite to chloride and molecular oxygen. In order to elucidate the role of conserved heme cavity residues in the catalysis of this reaction comprehensive mutational and biochemical analyses of Cld from "Candidatus

Higher phylogeny of zygaenid moths (Insecta: Lepidoptera) inferred from nuclear and mitochondrial sequence data and the evolution of larval cuticular cavities for chemical defence.

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Zygaenid moths are capable of releasing hydrogen cyanide in their defense by enzymatic break-down of cyanoglucosides, but only larvae of chalcosiine and zygaenine moths store cyanogenic compounds in cuticular cavities and thus are able to discharge defense droplets, which effectively deter potential

The H93G myoglobin cavity mutant as a versatile template for modeling heme proteins: ferrous, ferric, and ferryl mixed-ligand complexes with imidazole in the cavity.

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One of the difficulties in preparing accurate ambient-temperature model complexes for heme proteins, particularly in the ferric state, has been the generation of mixed-ligand adducts: complexes with different ligands on either side of the heme. The difference in the accessibility of the two sides of

Poly[tetraamminecopper(II) bis[tris(μ2-cyanido-κ2C,N)dicuprate(I)]]: a unique Cu(I)-Cu(II) mixed-valence complex containing anionic cuprous cyanide layers and [Cu(NH3)4]2+ cations.

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The title compound, {[Cu(NH(3))(4)][Cu(CN)(3)](2)}(n), features a Cu(I)-Cu(II) mixed-valence CuCN framework based on {[Cu(2)(CN)(3)](-)}(n) anionic layers and [Cu(NH(3))(4)](2+) cations. The asymmetric unit contains two different Cu(I) ions and one Cu(II) ion which lies on a centre of inversion.

Hydrogen cyanide in the headspace of oral fluid and in mouth-exhaled breath.

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Mouth-exhaled hydrogen cyanide (HCN) concentrations have previously been reported to originate from the oral cavity. However, a direct correlation between the HCN concentration in oral fluid and in mouth-exhaled breath has not been explicitly shown. In this study, we set up a new methodology to

Giant metal-cyanide coordination clusters: tetracapped edge-bridged cubic Cr(12)Ni(12)(CN)(48) and double face-centered cubic Cr(14)Ni(13)(CN)(48) species.

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The crystal structures of two new metal-cyanide clusters with record high nuclearities are reported. A direct assembly reaction involving [(Me(3)tacn)Cr(CN)(3)] (Me(3)tacn = N,N',N"-trimethyl-1,4,7-triazacyclononane), NiI(2), and KCN in aqueous solution affords

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