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Страница 1 от 35 полученные результаты
Salicylic Acid induces cyanide-resistant respiration in tobacco cell-suspension cultures.
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Cyanide-resistant, alternative respiration in Nicotiana tabacum L. cv Xanthi-nc was analyzed in liquid suspension cultures using O(2) uptake and calorimetric measurements. In young cultures (4-8 d after transfer), cyanide inhibited O(2) uptake by up to 40% as compared to controls. Application of 20
Cyanide-Resistant Respiration in Suspension Cultured Cells of Nicotiana glutinosa L.
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The respiration of dark-grown Nicotiana glutinosa L. cells in liquid suspension culture was found to be highly cyanide resistant and salicylhydroxamic acid (SHAM) sensitive, indicative of an active alternative respiratory pathway. This was especially true during the lag and logarithmic phases of the
Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog.
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Cyanoalanine hydratase (E.C. 4.2.1.65) is an enzyme involved in the cyanide detoxification pathway of higher plants and catalyzes the hydrolysis of beta-cyano-L-alanine to asparagine. We have isolated the enzyme from seedlings of blue lupine (Lupinus angustifolius) to obtain protein sequence
Development and enzyme activity of protein bodies in proteinoplasts of tobacco root cells.
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The development of protein bodies in proteinoplasts of tobacco (Nicotiana tabacum L. var. Wis. 38) roots was investigated with TEM, HVEM, and enzyme cytochemistry. These plastids contain a three-dimensional network of fenestrated tubules which originate from invaginations of the inner membrane of
Lack of mitochondrial and nuclear-encoded subunits of complex I and alteration of the respiratory chain in Nicotiana sylvestris mitochondrial deletion mutants.
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We previously have shown that Nicotiana sylvestris cytoplasmic male sterile (CMS) mutants I and II present large mtDNA deletions and that the NAD7 subunit of complex I (the main dehydrogenase of the mitochondrial respiratory chain) is absent in CMS I. Here, we show that, despite a large difference
Functional analysis reveals effects of tobacco alternative oxidase gene (NtAOX1a) on regulation of defence responses against abiotic and biotic stresses.
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Mitochondrial AOX (alternative oxidase) is the terminal oxidase of the CN (cyanide)-resistant alternative respiratory pathway in plants. To investigate the role of the tobacco AOX gene (NtAOX1a) (where Nt is Nicotiana tabacum) under deleterious conditions which could induce ROS (reactive oxygen
Amino Acid Transport in Suspension-Cultured Plant Cells : VI. Influence of pH Buffers, Calcium, and Preincubation Media on l-Leucine Uptake.
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The rate at which l-leucine was transported into suspension-cultured Nicotiana tabacum cv Wisconsin 38 cells increased more than 2-fold over a period of hours when the cells were preincubated in a 1% sucrose solution. This increase in uptake rate was eliminated if certain tris buffers were included
[Detection of trehalose in transgenic tobacco by HPLC with ELSD].
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The E. coli trehase synthalose gene(otsA) was transferred into Nicotiana tabacum mediated by Agrobacterium, but the method for detecting low concentration of trehalose in transgenic plant was not available. The high performance liquid chromatograph(HPLC) with evaporative light-scatting
Role of the Plasmalemma H-ATPase in Pseudomonas syringae-Induced K/H Exchange in Suspension-Cultured Tobacco Cells.
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Activation of a host plasma membrane K(+) efflux/net H(+) uptake exchange by pathogenic pseudomonads plays an important role in the development of hypersensitivity in tobacco (Nicotiana tabacum). Involvement of the plasmalemma H(+)-pumping ATPase in this response was investigated. The exchange
Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells.
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Some compounds that contain glucose groups can be transported across the plasma membrane into the cells through hexose transporters. To test the hypothesis that glucose-conjugated insecticides also have similar uptake and translocation properties, a novel fluorescent conjugate (12) was prepared by
Influence of ionic thiocyanate on growth of cabbage, bean, and tobacco.
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Plant weight and contents of chlorophyll, ionic thiocyanate (SCN(-)), and hydrogen cyanide (HCN) were determined in cabbage (Brassica oleracea var.capitula L. cv. Early Greenball), bean (Phaseolus vulgaris L. cv. Contender), and tobacco (Nicotiana tabacum L. cv. Delhi 76) grown hydroponically in
Mitochondrial alternative oxidase is involved in both compatible and incompatible host-virus combinations in Nicotiana benthamiana.
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The alternative oxidase (AOX) functions in the resistance to biotic stress. However, the mechanisms of AOX in the systemic antiviral defense response and N (a typical resistance gene)-mediated resistance to Tobacco mosaic virus (TMV) are elusive. A chemical approach was undertaken to investigate the
On the acceptor specificity of glycollate oxidase of Nicotiana tabacum.
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The effect of compounds on the activity of ammonium sulphate preparations of glycollate oxidase from Nicotiana tabacum cv. John Williams' Broadleaf and the aurea mutant Su/su is reported. Coupling to DCPIP as terminal oxidant under anaerobic conditions gave greater rates of glycollate oxidation than
High-level expression of alternative oxidase protein sequences enhances the spread of viral vectors in resistant and susceptible plants.
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The alternative oxidase (AOX) is the terminal oxidase of the cyanide-resistant alternative respiratory pathway in plants and has been implicated in resistance to viruses. When tobacco mosaic virus (TMV) vectors were used to drive very high levels of expression of either AOX or AOX mutated in its
Photorespiratory Properties of Mesophyll Protoplasts of Nicotiana plumbaginifolia.
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The photorespiratory activity of mesophyll protoplasts of Nicotiana plumbaginifolia has been clearly demonstrated by the presence of a Warburg-effect, the occurrence of an important CO(2)-sensitive O(2) uptake and the effect of some photorespiratory inhibitors on photosynthetic activity. At a
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