Antifungal properties of phosphatidylcholine-oleic acid liposomes encapsulating garlic against environmental fungal in wheat bread.

Liposomes have gained great interest in the food and pharmaceutical industry as colloidal carriers of bioactive compounds. In this work, liposomes of phosphatidylcholine (PC) and oleic acid (OA) encapsulating garlic extract (GE) were developed to determine its aptitude as antifungal agent in wheat bread. The influence of GE on the properties of liposomes were followed by determination of size, Zeta potential, Fourier transform infrared patterns (FTIR), morphology, differential scanning calorimetry (DSC) and thermogravimetric (TGA) techniques. The produced PC-OA-GE liposomes showed spherical morphology with narrow size distribution, entrapment efficiency of 79.7% and zeta potential of -27.9 mV. In vitro antifungal test showed noticeable inhibitory activities for free and encapsulated GE against selected fungal strains. TGA analysis revealed that the presence of OA and GE in the formulation retards the liposomal thermal decomposition, as compared with the pure PC liposomes and the DSC enthalpy and main transition temperature variation in PC-OA-GE liposomes suggested a strong heat-induced rigidifying effect that could be attributed to the presence of garlic polysaccharides in the liposome surface, observed by FTIR. In the in situ test, the bread formulations with free or liposome-encapsulated GE (0.65 mL/100 g of dough) were microbiologically more stable as compared with the controls, showing mold inhibition for five days. Therefore, liposomes formulated with OA and GE showed potential as natural antifungal agent in bakery products.