[Hypoxia and hyperglycemia promote the proliferation and migration of human marrow mesenchymal stem cells in vitro].
Kľúčové slová
Abstrakt
OBJECTIVE
To investigate the cell proliferation, apoptosis and migration of human marrow mesenchymal stem cells (hMSCs) under hypoxia and hyperglycemia.
METHODS
The hMSCs from healthy adults were isolated and harvested by density gradient centrifugation followed by adherent cultures. The cells was divided into four groups: control group (210 mL/L O2 with 5.56 mmol/L glucose), high glucose and normoxia group (210 mL/L O2 with 30 mmol/L glucose), normal glucose and hypoxia group (50 mL/L O2 with 5.56 mmol/L glucose) and high glucose and hypoxia group (50 mL/L O2 with 30 mmol/L glucose). The cells at the third passage were cultured in different groups. Then, we used cell counting kit-8 (CCK-8) to detect the proliferation of hMSCs, Transwell(TM) assay to examine the migration of hMSCs, and flow cytometry to observe the apoptosis of hMSCs at 24 hours.
RESULTS
Compared with the control group, the proliferation rate of hMSCs increased in the other groups at 24 and 48 hours, and the maximum proliferation effect was found in high glucose and hypoxia group. Compared with the control group, the migration ability of hMSCs in the other groups was enhanced dramatically at 24 hours, whereas there was no significant difference in the apoptosis rate among the four groups.
CONCLUSIONS
Both high glucose and hypoxia could improve the migration and proliferation of hMSCs, but they have no effect on apoptosis.
