Identification Of Two Arabidopsis thaliana Plasma Membrane Transporters Able To Transport Glucose 1-phosphate.
Kľúčové slová
Abstrakt
Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidence has shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose, and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. Here we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal a antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.